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【目的】阐明人X线修复交叉互补基因1(XRCC1)在人支气管上皮细胞DNA单链断裂修复(SSBR)中的作用。【方法】以XRCC1蛋白缺陷细胞株为模型,用标准断裂剂甲基磺酸甲酯(MMS)染毒,检测细胞存活、微核形成、彗星实验及细胞周期变化等方法测定细胞遗传毒性。【结果】XRCC1蛋白水平的降低使细胞对DNA损伤剂的杀细胞效应敏感度增加、微核形成增加、DNA单链损伤修复能力降低以及引起明显的细胞周期阻滞。【结论】结果提示XRCC1基因在DNA单链损伤修复及基因组稳定性方面起着重要的作用。
【Objective】 To elucidate the role of human X-ray repair cross-complementary gene 1 (XRCC1) in DNA single-strand break repair (SSBR) in human bronchial epithelial cells. 【Method】 Cytotoxicity was determined by XRCC1 protein-deficient cell strain model with standard methylmethanesulfonate (MMS), cell survival, micronucleus formation, comet assay and cell cycle changes. 【Results】 The decrease of XRCC1 protein level increased the sensitivity of cells to cytotoxicity of DNA damaging agents, increased the formation of micronuclei, decreased the capacity of single-strand DNA damage repair and caused obvious cell cycle arrest. 【Conclusion】 The results suggest that XRCC1 gene plays an important role in single-strand DNA damage repair and genomic stability.