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目的:建立基于肠吸收技术以快速发现白芍吸收成分群的方法,为选择白芍质量控制指标提供参考。方法:采用大鼠肠外翻模型,收集3个质量浓度白芍提取物给药后不同时间的肠囊液样品,用HPLC进行检测分析,并计算其有效成分芍药内酯苷、芍药苷的累计吸收量。结果:白芍中5种主要成分均可进入肠囊,在45 min后能全部检测到;不同质量浓度白芍中芍药内酯苷、芍药苷在各肠段均为线性吸收,R2均大于0.9,符合零级吸收速率;芍药内酯苷和芍药苷在空肠、回肠中的吸收速率常数(Ka)随着白芍提取物浓度增加而增加(P<0.05),符合被动吸收。结论:基于肠吸收的质量评价指标选择方法(SEMAC)可以用于白芍吸收成分群的研究。肠囊对药物成分吸收有选择性,回肠与空肠相比可更多、更快地给出吸收成分信息,选择肠外翻60 min的样品检测白芍吸收成分比较适宜。
OBJECTIVE: To establish a method based on intestine absorption technology to rapidly detect the absorption components of Radix Paeoniae Alba, and provide references for the quality control of Radix Paeoniae Alba. Methods: The rat intestinal enteral eversion model was used to collect samples of enteric cyst fluid at different time points after the administration of the extract of Radix Paeoniae Alba, and its content was determined by HPLC. The cumulative contents of the active constituents albiflorin and paeoniflorin were calculated Absorption. Results: The main constituents of Radix Paeoniae Alba could enter into the gut capsule and all of them could be detected after 45 min. The concentrations of Paeoniflorin and Paeoniflorin in different concentrations of Radix Paeoniae Alba were linearly absorbed in all intestine segments, R2 were all above 0.9 , Which accorded with the zero-order absorption rate. The absorption rate constant (Ka) of peoninide and paeoniflorin in jejunum and ileum increased with the increase of the concentration of peony root extract (P <0.05), which accorded with passive absorption. Conclusion: The method of SEMAC based on intestine absorption can be used to study the absorption group of Paeonia. Intestinal sac on the absorption of the drug composition is selective, ileum and jejunum compared to more and more to give information on the absorption components, the choice of parenteral 60 min sample detection of peony root absorption component more appropriate.