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目的 :探讨乳腺癌组织中 β -连接素( β catenin)、细胞周期蛋白D1(cyclinD1)的表达与乳腺癌发生、浸润及转移的关系。方法 :应用SP免疫组化方法检测 60例乳腺癌组织中 β catenin、cyclinD1的表达。 结果 :乳腺癌组织中有 42例 ( 70 % ) β catenin异常表达 ,2 8例( 4 6 7% )cyclinD1过度表达。β catenin异常表达率与乳腺癌大小、分化程度及淋巴结转移与否无显著相关性 ,P >0 0 5 ;β catenin在Ⅲ～Ⅳ期乳腺癌、浸润性小叶癌中的异常表达率( 90 %、95 % )明显高于Ⅰ～Ⅱ期乳腺癌、浸润性导管癌 ( 60 %、5 8 3 % ) ,P <0 0 5 ,P <0 0 1。cyclinD1过度表达率与乳腺癌大小、组织学类型、分化程度无显著相关性 ,P >0 0 5 ;与TNM分期、淋巴结转移与否有显著相关性 ,P <0 0 5。β catenin异常表达病例中 ,5 7 1%( 2 4/4 2 )的病例呈现cyclinD1的过度表达 ,但β catenin正常膜表达病例中 ,2 2 2 % ( 4 /18)的病例呈现cyclinD1的过度表达。β catenin异常表达与cyclinD1过度表达有显著的正相关性 ,rs=0 3 2 1,P <0 0 5。结论 :β catenin的异常表达激活cyclinD1的过度表达 ,引起细胞增殖和分化失控 ,在乳腺癌的发生中可能起着重要作用。β catenin的异常表达不是影响乳腺癌细胞转移的主要因素 ,但可?
Objective: To investigate the relationship between the expression of β - catenin and cyclin D1 in breast cancer, infiltration and metastasis of breast cancer. Methods: The expression of β catenin and cyclinD1 in 60 cases of breast cancer was detected by SP immunohistochemistry. Results: There were 42 (70%) β catenin abnormalities in breast cancer tissues and 28 (46.7%) cyclin D1 overexpression. There was no significant correlation between the abnormal expression of β catenin and the size, differentiation and lymph node metastasis of breast cancer (P> 0.05). The abnormal expression of β catenin in stage Ⅲ ~ Ⅳ breast cancer and invasive lobular carcinoma (90% , 95%) were significantly higher than those of stage Ⅰ ~ Ⅱ breast cancer and invasive ductal carcinoma (60%, 583%), P <0.05, P <0.01. There was no significant correlation between the overexpression rate of cyclinD1 and the size, histological type and differentiation of breast cancer (P> 0.05). There was a significant correlation between the overexpression of cyclinD1 and TNM stage and lymph node metastasis (P <0.05). In the cases with abnormal expression of β-catenin, cyclinD1 was found in 51.7% (24/42) of the cases, but in 22.3% of normal β-catenin cases, 22.2% (4/18) showed cyclinD1 overexpression expression. There was a significant positive correlation between abnormal expression of β catenin and overexpression of cyclinD1, rs = 0 3 2 1, P <0 05. Conclusion: Aberrant expression of β catenin activates overexpression of cyclinD1, which leads to uncontrolled cell proliferation and differentiation. It may play an important role in the development of breast cancer. Abnormal expression of β catenin is not a major factor affecting the metastasis of breast cancer cells, but can?