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亲和超滤HPLC-MS法是将超滤技术与HPLC-MS联用,主要用于从组合化学库和天然产物粗提物中筛选与可溶性靶点相结合的小分子活性物质,在药物代谢和描述受体-配体结合特征两方面也有所应用。用该方法筛选和发现药物被认为是传统药物发现方法的一种补充。本文综述了亲和超滤HPLC-MS法的原理及其在药物研究中的应用。
The affinity and ultrafiltration HPLC-MS method is a combination of ultrafiltration and HPLC-MS. It is mainly used to screen small molecule active substances that combine with soluble targets from crude chemical extracts of combinatorial chemical libraries and natural products. And describes receptor-ligand binding characteristics are also two applications. Screening and discovering drugs using this method is considered as a supplement to traditional drug discovery methods. This article reviews the principle of affinity ultrafiltration HPLC-MS and its application in drug research.