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目的 探讨使用微气量小动物呼吸机提高小鼠全脑缺血 /再灌注损伤模型的质量的可能性。方法 采用小白鼠双侧颈总动脉合并颈部软组织加压后再解压的方法复制小鼠全脑缺血 /再灌注损伤模型。并于加压前给小动物呼吸机进行人工通气 ,观察心电图、脑电图及病理学变化。结果 加压前使用小动物人工呼吸机维持生理必须通气量 ,各组动物在观察期间内心电图未见明显变化 ,不同的缺血和再灌注期间脑电图变平 ,未见恢复。病理组织学提示 :大脑皮质和海马随缺血和再灌注时间延长损害进一步加重。结论 使用微气量小动物呼吸机可提高小鼠全脑缺血 再灌注损伤模型的成功率和稳定性 ,降低动物死亡率。
Objective To explore the possibility of using micro-aerosol ventilator to improve the quality of mouse model of global cerebral ischemia-reperfusion injury. Methods The mouse model of global cerebral ischemia / reperfusion was established by bilateral carotid artery combined with decompression of the neck soft tissue. Ventilation of small animal ventilator was performed before pressurization, and ECG, EEG and pathological changes were observed. Results Before use of the small animal respirator to maintain physiological essential ventilation, the animals in each group during the observation ECG showed no significant change in different periods of ischemia and reperfusion EEG flattening, no recovery. Histopathology suggests that the damage to the cerebral cortex and hippocampus further aggravates with the prolongation of ischemia and reperfusion time. Conclusion The use of micro-aerosol ventilator can improve the success rate and stability of mouse model of global cerebral ischemia-reperfusion injury, reduce animal mortality.