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目的:探讨Msx2、topoⅡ-α、HPV16及VEGF在鼻内翻性乳头状瘤(SNIP)组织中的定量及意义,研究上述四种因子之间是否具有相关性,进一步确定Msx2和topoⅡ-α在SNIP恶变发生、发展中的关系。方法:采用实时荧光定量聚合酶链反应(RT-qPCR)分别检测Msx2、topoⅡ-α、VEGF及HPV16在13例SNIP,10例鼻息肉(INP),10例鼻腔-鼻窦鳞状细胞癌组织(NSCC)中的表达。其中SNIP根据病理形态分为轻度不典型增生组、中度不典型增生组和重度不典型增生组。结果:SNIP和NSCC组织中Msx2、topoⅡ-α、HPV16及VEGF的表达均显著高于INP(P<0.05)。Msx2、topoⅡ-α、VEGF及HPV16在SNIP的3个病理形态组之间的表达差异均有统计学意义(P<0.05)。采用Pearson相关系数得出HPV16、topoⅡ-α、VEGF、Msx2之间两两比较均呈正相关性(P<0.05)。结论:Msx2和topoⅡ-α在SNIP恶变的发生、发展中起重要作用,有可能成为SNIP和NSCC基因治疗的新靶点。
Objective: To investigate the quantitative and significance of Msx2, topoⅡ-α, HPV16 and VEGF in nasal inverted papilloma (SNIP) tissues and to investigate whether these four factors are correlated and further to determine whether Msx2 and topoⅡ- SNIP malignant transformation, the development of the relationship. Methods: The expressions of Msx2, topoⅡ-α, VEGF and HPV16 in 13 cases of SNIP, 10 cases of nasal polyps (INP) and 10 cases of nasal sinus tissue were detected by real-time fluorescence quantitative polymerase chain reaction NSCC). Among them, SNIP was classified into mild atypical hyperplasia group, moderate atypical hyperplasia group and severe atypical hyperplasia group according to the pathological changes. Results: The expressions of Msx2, topoⅡ-α, HPV16 and VEGF in SNIP and NSCC tissues were significantly higher than those in INP (P <0.05). The expressions of Msx2, topoⅡ-α, VEGF and HPV16 in three pathological SNIP groups were all significantly different (P <0.05). Pearson correlation coefficient was used to draw a positive correlation between HPV16, topoⅡ-α, VEGF and Msx2 (P <0.05). Conclusion: Msx2 and topoⅡ-α play an important role in the occurrence and development of SNIP malignant transformation, which may become a new target of SNIP and NSCC gene therapy.