论文部分内容阅读
目的:研究酒精诱导的小鼠肝损伤过程中肝脏增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达变化.方法:将100只健康清洁♂小鼠随机分为对照组(n=40)和模型组(n=60),模型组小鼠每日给予56%的红星二锅头(10 m L/kg)灌胃,对照组每日给予等体积的蒸馏水灌胃.分别于第1、2、3、4周将两组小鼠全部处死,并在各时间点取小鼠肝脏做苏木精-伊红(HE)染色并测定血清中ALT酶活力,以检测小鼠酒精性肝损伤程度.剩余肝脏通过蛋白印迹法用以检测小鼠肝脏增殖细胞核抗原的表达情况.应用SSPS16.0对实验数据进行ANOVA显著性分析和Duncan’s test.结果:酒精灌胃第1周,病理HE染色显示肝细胞轻度损伤,ALT酶活力显著上升,小鼠肝脏中的PCNA的表达量相比正常小鼠显著下降(P<0.05);第2周,病理HE染色显示肝细胞出现明显的水肿和大量的炎细胞浸润,ALT酶活力高于正常,PCNA的表达量达到了最低值(P<0.01);第3周,病理HE染色显示中央静脉周围的肝细胞脂肪变性,门管区周围肝细胞水样变,ALT酶活力下降,PCNA的表达量显著上升,但仍低于正常小鼠肝脏PCNA的表达量(P<0.05).
Objective: To study the changes of hepatic proliferating cell nuclear antigen (PCNA) in alcohol-induced liver injury.Methods: 100 healthy and clean male mice were randomly divided into control group (n = 40) and model (N = 60) .Model group mice were given gavage of 56% red ganoderma (10 m L / kg) daily, and the control group were given equal volume of distilled water by gavage respectively.At the same time, The mice in both groups were sacrificed at 4 weeks, and the liver of mice was sacrificed at each time point for hematoxylin-eosin (HE) staining and the activity of ALT in serum was measured to detect the extent of alcoholic liver injury in mice. Western blotting was used to detect the expression of proliferating cell nuclear antigen (PCNA) in mouse liver.Analysis of the significance of ANOVA and Duncan’s test were performed on the experimental data by SSPS16.0.Results: In the first week of alcohol administration, pathological HE staining showed mild The expression of PCNA in liver decreased significantly compared with normal mice (P <0.05). At the second week, pathological HE staining showed obvious edema and inflammatory cell infiltration in hepatocytes , ALT enzyme activity was higher than normal, PCNA expression reached the lowest (P <0.01). In the third week, the histopathological HE staining showed steatosis of hepatic cells around the central vein, changes of watery hepatocytes and ALT activity in the portal area, and the expression of PCNA increased significantly, but still lower than the normal Rat liver PCNA expression (P <0.05).