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目的：测定中药胎宝对生殖免疫的作用。方法：将人精子膜抗原注入雌性小鼠腹腔，测定精子抗体为阳性者确定为免疫不育动物模型。分别灌胃中药胎宝高剂量（４６．８ｇ·ｋｇ－１·ｄ－１）、低剂量（３１．２ｇ·ｋｇ－１·ｄ－１），并以强的松（６ｍｇ·ｋｇ－１·ｄ－１）和生理盐水作对照，观察胎宝抑制实验小鼠抗精子抗体、促进妊娠的作用。结果：生理盐水组受孕率３８．８９％，强的松组４７．０６％，胎宝高、低剂量组分别为７０．００％、７５．００％。胎宝低剂量组与生理盐水组比较有显著性差异（Ｐ＜０．０５）。胎宝低剂量组孕鼠平均着床数（１０．９３±２．９１）明显高于强的松组（６．７５±４．５９）。酶联免疫吸附试验（ＥＬＩＳＡ）和微量细胞毒试验测定抗精子抗体，显示胎室组精子抗体下降，胎宝高剂量组和强的松组细胞毒性百分率均显著低于生理盐水组（Ｐ＜０．０５，Ｐ＜０．０１）。结论：中药胎宝对生殖免疫有调节作用，能抑制精子细胞毒抗体，从而促进免疫不育小鼠生育力。
Objective: To determine the effect of Chinese medicine Tibao on reproductive immunity. Methods: The human sperm membrane antigen was injected into the abdominal cavity of female mice. The determination of sperm antibody was positive and it was determined as animal model of immune infertility. High-dose (46.8g·kg-1·d-1) and low-dose (31.2g·kg-1·d-1) of traditional Chinese medicine Tibao, and prednisone (6mg·kg-1·) D-1) and saline were used as controls to observe the effect of fetal treasure in inhibiting anti-sperm antibodies in experimental mice and promoting pregnancy. Results: The pregnancy rate was 38.89% in the saline group, 47.06% in the prednisone group, and 70.00% in the fetal treasure group and 75.00% in the low dose group. There was a significant difference between the low-dose Tibao group and the saline group (P<0.05). The average number of implantations in the low-dose group of fetal treasure (10.93±2.91) was significantly higher than that of the prednisone group (6.75±4.59). Enzyme-linked immunosorbent assay (ELISA) and micro-cytotoxicity assays were used to determine anti-sperm antibodies, showing that the serum antibody levels in the child-to-child group decreased, and the percentage of cytotoxicity in the high-dose group and prednisone group was significantly lower than that in the saline group (P<0). .05, P<0.01). Conclusion: Chinese medicine fetal treasure has a regulatory effect on reproductive immunity, can inhibit sperm cytotoxic antibodies, and thus promote fertility in infertile mice.