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目的探讨核输入受体蛋白13(Importin 13,IPO13)在子宫内膜异位症和子宫内膜癌中的表达及其临床意义。方法随机选取20份正常增殖期子宫内膜和20份正常分泌期子宫内膜标本为对照组A、B,20例子宫内膜异位症和20例子宫内膜癌患者的子宫内膜标本为实验组C、D。采用免疫组化法检测IPO13在各组中是否表达;免疫荧光法检测IPO13和干/祖细胞标记在各组中的共表达;荧光定量PCR法检测各组中IPO13基因mRNA转录水平;Western blot法检测IPO13蛋白在各组中的表达水平。结果 IPO13在各组子宫内膜腺上皮细胞和间质细胞的胞浆及胞核中均有表达。对照组B的IPO13阳性细胞数明显低于对照组A和实验组(P<0.01)。IPO13与CD34、CD45、端粒酶、OCT4、C-KIT、PDGF-Rβ、CD90和CD146共表达于各组子宫内膜的上皮细胞和间质细胞;实验组及对照组A的IPO13基因mRNA和IPO13蛋白表达量明显高于对照组B(P<0.01)。结论 IPO13在正常子宫内膜中有表达,且增殖期表达高于分泌期;在子宫内膜异位症和子宫内膜癌中的表达明显高于正常分泌期子宫内膜,此结果可能与子宫内膜干/祖细胞的表达异常相关;IPO13可作为子宫内膜干/祖细胞的一个候选的标记。
Objective To investigate the expression of nuclear import receptor 13 (IPO13) in endometriosis and endometrial carcinoma and its clinical significance. Methods 20 samples of normal proliferative endometrium and 20 normal secretory endometrium were randomly selected as the control group A, B, 20 cases of endometriosis and 20 cases of endometrial cancer endometrial specimens were Experimental group C, D. Immunohistochemistry was used to detect IPO13 expression in each group; Immunofluorescence method was used to detect the co-expression of IPO13 and stem / progenitor cells in each group; Fluorescence quantitative PCR was used to detect the transcription level of IPO13 gene in each group; Western blot The IPO13 protein levels in each group were measured. Results IPO13 was expressed in the cytoplasm and nucleus of all groups of endometrial glandular epithelial cells and stromal cells. The number of IPO13 positive cells in control group B was significantly lower than that in control group A and experimental group (P <0.01). IPO13 and CD34, CD45, telomerase, OCT4, C-KIT, PDGF-Rβ, CD90 and CD146 were co-expressed in the endometrial epithelial cells and stromal cells in each group; IPO13 gene mRNA and IPO13 protein expression was significantly higher than the control group B (P <0.01). Conclusions IPO13 is expressed in normal endometrium and proliferative phase is higher than in secretory phase. The expression of IPO13 in endometriosis and endometrial carcinoma is significantly higher than that in normal endometrial secretion, which may be related to the uterus The expression of endomembrane stem / progenitor cells is abnormally correlated; IPO13 serves as a candidate marker for endometrial stem / progenitor cells.