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目的 :观察局部缓释秋水仙素 (colchicine ,COL)对移植血管内膜增生的抑制程度。方法 :采用大鼠自体静脉移植模型 ,外涂含不同浓度COL的Pluronic胶 ,于术后 14d处死取材 ,行HE ,Verhoeff弹力纤维染色、增殖细胞核抗原 (proliferatingcellularnuclearantigen ,PCNA)免疫组化染色 ,测量内膜厚度、内膜与中膜面积比、PCNA阳性细胞百分比 ,用流式细胞仪计数各期细胞百分比 ,观察不同浓度COL对内膜及平滑肌细胞增殖的抑制程度。结果 :血管移植术后 14d ,对照组的内膜厚度、内膜面积与中膜面积比 ,与高浓度组、中浓度组、低浓度组相比 ,差异显著(P <0 .0 5 )。PCNA阳性细胞数在各组之间相互比较 ,差异显著 (P <0 .0 5 )。流式细胞仪分析 ,从高浓度组、中浓度组、低浓度组到对照组 ,G0 G1、S期细胞所占比例逐渐减少 ,G2 M期细胞所占比例逐渐增多 (P <0 .0 5 )。结论 :局部缓释COL具有抑制移植血管内膜及平滑肌细胞增殖的作用。COL抑制血管内膜增殖作用与其剂量呈剂量—效应关系。COL可使平滑肌细胞停止在分裂中期 ,抑制其进入下一个增殖周期 ,从而抑制移植血管内膜及平滑肌细胞增殖的作用。
Objective: To observe the inhibitory effect of colchicine (COL) on the intimal hyperplasia of graft. Methods: Autologous vein graft model was adopted in rats. Pluronic gum with different concentration of COL was applied to the rats and sacrificed 14 days after operation. HE, Verhoeff elastin staining and proliferating cell nuclear antigen (PCNA) immunohistochemical staining were performed. Membrane thickness, ratio of intima to media area and percentage of PCNA positive cells. The percentage of cells in each phase was counted by flow cytometry. The inhibitory effect of different concentrations of COL on the proliferation of intima and smooth muscle cells was observed. Results: At 14 days after vascular graft, the intima-media thickness, intima-media area ratio in control group were significantly different from those in high-concentration group, middle-concentration group and low-concentration group (P <0.05). PCNA positive cells in each group compared with each other, the difference was significant (P <0. 05). Flow cytometry analysis showed that the percentage of G0 G1 and S phase cells gradually decreased and the proportion of G2 M phase cells gradually increased from high concentration group, middle concentration group and low concentration group to the control group (P <0.05) ). CONCLUSION: Local sustained release of COL can inhibit the proliferation of vascular intima and smooth muscle cells. COL inhibited the proliferation of intima and its dose dose-response relationship. COL can stop smooth muscle cells in the metaphase, inhibit its entry into the next cycle of proliferation, thereby inhibiting the proliferation of vascular intima and smooth muscle cell transplantation.