应用离体细胞不同组合分析法来研究获得性低γ球蛋白血症的多种淋巴细胞缺损

• 作 者: 刘尔翔
• 出 处: 国外医学(免疫学分册)
• 发表日期: 1981年04期
• 关键词: 羊红细胞 Ficoll 组合分析 bromide γ球蛋白 玫瑰花 单核细胞 离体细胞 细胞形成 血症

病人周围血液用Ficoll-Hypaque液分离单核细胞,再用S-(2-Aminoethyl)isothiouronium bromide hydrobromide(AET)处理的羊红细胞与T细胞形成玫瑰花而分别得到T细胞与B细胞。将用上法分离的正常人与病人的B细胞、T细胞和经放

Peripheral blood of patients was isolated from mononuclear cells by Ficoll-Hypaque solution and sheep erythrocytes treated with S- (2-Aminoethyl) isothiounonium bromide hydrobromide (AET) formed T-cells and rose cells to obtain T cells and B cells, respectively. B cells, T cells, and normal cells isolated from normal and patient will be separated