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目的 探讨肾癌与高危型人乳头状瘤病毒基因 (HPV1 6 ,1 8DNA)的相关性。方法 采用聚合酶链反应 (PCR)和DNA限制性内切酶酶切技术 (DNARET)对 90例成人肾恶性肿瘤组织 (其中肾透明细胞癌、肾乳头状癌、肾颗粒细胞癌各 30例 )的HPV1 6、1 8E7基因进行实验研究 ,2 0例正常肾脏组织为正常对照 ,HPV1 6、1 8阳性克隆作为阳性对照 ,去离子三蒸水做实验体系阴性对照。对每一阳性标本行限制性内切酶技术。结果 肾透明细胞癌、肾乳头状癌、肾颗粒细胞癌中HPV1 6基因表达率分别为 60 .0 %、86 .7%、63 .3 % ,总的基因表达率为 70 .0 %。HPV1 8的基因阳性率分别为 46 .7%、66 .7%、44 .3 % ,总的基因表达率为 52 .2 %。结论 高危型HPV1 6、1 8DNA与肾恶性肿瘤有一定的关系 ,尤其是HPV1 6基因表达率明显高于HPV1 8型 (P <0 .0 5) ,其中肾乳头状癌的HPV1 6、1 8基因阳性率明显高于肾透明细胞癌和肾颗粒癌 (P <0 .0 5) ,证实了高危型人乳头状瘤病毒感染是肾癌的重要致癌因素之一
Objective To investigate the relationship between renal cell carcinoma and high risk human papilloma virus (HPV16, 18 DNA). Methods 90 cases of adult renal malignant tumor tissues (including clear cell renal cell carcinoma, papillary renal cell carcinoma and renal granulosa cell carcinoma) were studied by polymerase chain reaction (PCR) and DNA restriction endonuclease digestion (DNARET) Of HPV16, 18E7 genes in the experimental study, 20 normal kidneys as a normal control, HPV16,180 positive clone as a positive control, deionized three distilled water to do the experimental system negative control. Restriction enzyme digestion was performed on each positive specimen. Results The positive rates of HPV16 in renal clear cell carcinoma, renal papillary carcinoma and renal cell carcinoma were 60.0%, 86.7% and 63.3%, respectively. The total gene expression rate was 70.0%. The positive rate of HPV18 was 46.7%, 66.7% and 44.3% respectively, and the total gene expression rate was 52.2%. Conclusions High-risk HPV16 DNA is associated with malignant neoplasm. In particular, the expression of HPV16 is significantly higher than that of HPV18 (P <0.05), and HPV16, 18 Gene positive rate was significantly higher than that of renal clear cell renal cell carcinoma and renal cell carcinoma (P <0.05), confirming the high risk of human papillomavirus infection is one of the important carcinogenesis of renal cell carcinoma