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目的检测缺氧诱导因子1α(hypoxia-inducible factor 1α,HIF-1α)和2型谷氨酰胺酶(glutaminase 2,GLS2)在结直肠癌中的表达,探讨其临床意义。方法应用免疫组化法检测本院结直肠癌术后108例组织标本(其中23例含癌旁黏膜组织)中HIF-1α和GLS2的表达,分析二者的表达与临床病理参数的相关性;采用Spearman等级相关分析检测HIF-1α和GLS2表达的相关性,Kaplan-Meier法分析结直肠癌中HIF-1α和GLS2的表达与患者预后的关系,Cox回归模型分析结直肠癌患者预后的影响因素。结果结直肠癌组织中HIF-1α和GLS2的表达均高于癌旁黏膜组织,差异有统计学意义(P<0.01)。HIF-1α的表达与结直肠癌患者年龄、TNM分期、淋巴结转移、术后远处转移密切相关(P<0.05),而GLS2的表达与结直肠癌患者年龄、浸润深度、术后远处转移密切相关(P<0.05)。HIF-1α和GLS2的表达显著正相关(r=0.464,P<0.01),且均与结直肠癌的预后有关,Cox回归分析显示TNM分期、术后远处转移及HIF-1α、GLS2的表达均是结直肠癌预后的独立影响因素(P<0.05)。结论结直肠癌患者中HIF-1α、GLS2高表达预后均较低表达差,提示二者在结直肠癌的发生、发展中可能发挥重要作用。
Objective To investigate the expression of hypoxia-inducible factor 1α (HIF-1α) and glutaminase 2 (GLS2) in colorectal cancer and to explore its clinical significance. Methods The expression of HIF-1α and GLS2 in 108 cases of colorectal cancer tissues (including 23 cases of paracancerous mucosa) were detected by immunohistochemistry. Correlation between the expression of HIF-1α and clinicopathological parameters was analyzed. Spearman rank correlation analysis was used to detect the correlation between HIF-1α and GLS2 expression. The Kaplan-Meier method was used to analyze the relationship between the expression of HIF-1α and GLS2 and the prognosis of patients with colorectal cancer. Cox regression model was used to analyze the prognostic factors of colorectal cancer . Results The expressions of HIF-1α and GLS2 in colorectal cancer tissues were significantly higher than those in para-cancerous mucosa (P <0.01). The expression of HIF-1α was closely related to age, TNM stage, lymph node metastasis and distant metastasis in patients with colorectal cancer (P <0.05), while the expression of GLS2 was correlated with age, depth of invasion, distant metastasis Closely related (P <0.05). There was a significant positive correlation between the expression of HIF-1α and GLS2 (r = 0.464, P <0.01), and both were correlated with the prognosis of colorectal cancer. Cox regression analysis showed that TNM stage, distant metastasis and expression of HIF-1α and GLS2 Are independent prognostic factors of colorectal cancer (P <0.05). Conclusion The low expression of HIF-1α and GLS2 in colorectal cancer patients is poorly expressed, which may play an important role in the occurrence and development of colorectal cancer.