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The objective of this experiment is to evaluate the role of intracellular and extracellular Ca2+ and calmodulin (CaM) in jasmonic acid (JA) signaling. The laser scanning microscopy was used to detect the changes of [Ca2+]cyt of Arabidopsis thaliana leaf cells which pretreated with different types of calcium channel blocker. Moreover, the expression of VSP, one of JA response genes, was also investigated after pretreated with the above blocker and antagonist of CaM. The results showed that extracellular and intracellular calcium both involved in the JA-induced Ca2+ mobilization, and then Ca2+ exerted its functions through activating the CaM or CaM related proteins. The apoplast calcium influx and the calcium release from the calcium stores are both involved in the JA-induced calcium mobilization, then the JA-induced Ca2+ transmited the JA signal through CaM or CaM related proteins, and regulated the JA responsive genes.