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本试验对蚕豆进行染色体Ag—NOR(银染核仁组成区)技术的研究,采用根尖压片,短时间固定,AgNO3染色,Giemsa复杂,最后得到2个黑色的核仁组成区,证明蚕豆组型中有2处NOR,这和蚕豆核型是一致的,此方法可以广泛应用于次缢痕(或NOR)难以确定的核型研究中。
In this study, we studied the technique of Ag-NOR (silver-stained nucleolar organizer) region of Vicia faba. The apical tablet, short-term fixation, AgNO3 staining and Giemsa complex were used to study the structure of Vicia faba. There are two NORs in the group, which is consistent with the faba bean karyotype. This method can be widely used in the karyotype studies where the secondary marks (or NOR) are difficult to determine.