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采用8M尿素和简化方法分离、提取斯氏狸殖吸虫成虫尿素溶解性抗 原(PsAWU),并对该抗原和水溶性抗原(PsAWA)的血清学特性进行了分析比较,结果表明PsAIVU的敏感性高于PsAWA,初步证明具一定的特异性,是一种可实际应用的新型抗原。为进一步分析两种抗原,对其蛋白组成及活性成份进行了初步研究。 氨基酸组成的分析 两种抗原浓度均为1 mg/ml,用稀盐酸加热处理后,以氨基酸分析仪(Beckman公司)进行测定。结果表明两种抗原的氨基酸种类基本一致,但绝大
The isolation and extraction of PsAWU by 8M urea and simplified method were used to analyze the serological characteristics of PsAWA. The results showed that the sensitivity of PsAIVU was high At PsAWA, initially proved to have a certain specificity, is a practical application of new antigens. To further analyze the two antigens, the protein composition and active ingredients were studied. Analysis of Amino Acid Compositions The concentrations of both antigens were 1 mg / ml, which were measured with an amino acid analyzer (Beckman) after heat treatment with dilute hydrochloric acid. The results showed that the two kinds of antigen amino acid species are basically the same, but vast