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从溶液中回收或精制胰岛素最常用的方法是结晶法。一般是采用锌盐结晶法,而胰岛素钠盐结晶的制备国内还未见报导。据Jackson报导,可在胰腺酸醇粗提液中直接采用这种结晶法,收率每公斤胰腺可达2600～4600IU。我们利用层析法去除二价离子后的猪胰岛素溶液,进行钠盐结晶制备的探索。实验部分基本步骤:去二价阳离子猪胰岛素溶液加NaOH调pH为7.2～10.0,搅拌下加入NaCl使溶液Na~+总浓度在0.2～1.0M范围内,室温下机械搅拌0.25～72小时,分离结晶体,母液回收重复上述步骤。
The most common method of recovering or refining insulin from solution is the crystallization method. The general use of zinc salt crystallization method, and the preparation of sodium salt of insulin has not been reported in the country. According to Jackson reports, this method can be used directly in the pancreatic acid alcohol crude extract, yield up to 2600 ~ 4600IU per kilogram of the pancreas. We use the chromatographic method to remove the porcine insulin solution after divalent ions to explore the preparation of sodium salt crystals. Experimental part of the basic steps: go to the divalent cation porcine insulin solution plus NaOH pH was adjusted to 7.2 to 10.0, with stirring NaCl was added to make the total concentration of Na ~ + in the range of 0.2 ~ 1.0M, stirred at room temperature for 0.25 ~ 72 hours, separated Crystals, mother liquor recovery Repeat the above steps.