诺美孕酮醋酸盐对离体培养大鼠卵巢颗粒细胞分泌甾体激素的抑制作用(英文)

• 作 者: 钱立晖 杨波 冷颖 曹霖 顾芝萍
• 机 构: 中国科学院上海生命科学研究院上海药物研究所,中国科学院上海生命科学研究院上海药物研究所,中国科学院上海生命科学研究院上海药物研究所,中国科学院上海生命科学研究院上海药物研究所,中国科学院上海生命科学研
• 出 处: Acta Pharmacologica Sinica
• 发表日期: 2001年01期
• 关键词: 诺美孕酮 卵巢颗粒细胞 抑制作用 离体培养 大鼠 培养的细胞 抑制率 可信限 活细胞计数 活细胞数

目的:观察诺美孕酮对离体培养大鼠颗粒细胞分泌雌、孕激素功能的抑制作用。方法:台盼蓝排斥法进行活细胞计数。加入FSH和睾酮刺激颗粒细胞激素分泌。放免法测定培养液中雌、孕激素含量。结果:诺美孕酮杀伤细胞的IC_(50)为6.85mg/L(95％可信限:5.36-8.75mg/L)。诺美孕酮0.45,0.9,和1.8mg/L在不影响活细胞数的情况下对颗粒细胞分泌雌激素的抑制率分别为7.6％,12.5％和28.3％,对其分泌孕激素的抑制率分别为44.5％,53.3％和62.0％。结论:诺美孕酮直接抑制离体培养的大鼠颗粒细胞分泌雌、孕激素。

Objective: To observe the inhibitory effect of nomegestrol on the secretion of estrogen and progesterone by cultured granulosa cells in vitro. Methods: Trypan blue exclusion method for viable cell count. FSH and testosterone are added to stimulate granulocyte hormone secretion. Radioimmunoassay determination of estrogen and progesterone in culture solution. Results: The IC50 of nomegestrin killer cells was 6.85 mg / L (95% confidence interval: 5.36-8.75 mg / L). The inhibitory rates of nomethindrone 0.45, 0.9, and 1.8 mg / L on the secretion of estrogen by granulosa cells without affecting the number of viable cells were 7.6%, 12.5% ​​and 28.3%, respectively. The inhibitory rate of progestin secretion Respectively 44.5%, 53.3% and 62.0%. CONCLUSION: Novolin progesterone directly inhibits the secretion of estrogen and progesterone by cultured rat granulosa cells.