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取臭椿[Ailanthus altissima(Mill.)Swingle]种子,用含三硝基甲苯-X-100的2%次氯酸钠消毒后,置陪替氏培养皿内使发芽,将萌发的无菌子叶苗移至含2,4-D1 mg/ml、激动素0.1 mg/l、1%聚乙烯吡咯烷酮和5%蔗糖的1%琼脂MS 固体培养基上,保持25°下光照培养,每4周换一次培养基。取第12周所得愈伤组织放入含50 ml 液体培养基的三角烧瓶中,在100rpm 振动下混悬培养,保持25°、光照,每2周换一次培养基,收集第8周所得的混悬培养物。除去培养液,
The seeds of Ailanthus altissima (Mill.) Swingle were taken and sterilized with 2% sodium hypochlorite containing Trinitrotoluene-X-100. After germination was performed in a petri dish, the germinated sterile cotyledon was moved to 2,4-D1 mg / ml, kinetin 0.1 mg / l, 1% polyvinylpyrrolidone and 5% sucrose in 1% agar MS solid medium maintained at 25 ° under light culture, the medium was changed every 4 weeks. The callus obtained in the twelfth week was placed in an Erlenmeyer flask containing 50 ml of liquid medium, suspended at 100 rpm under shaking, maintained at 25 °, light, the medium was changed every two weeks, collected the first 8 weeks Suspension culture. Remove the culture medium,