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应用间接免疫荧光法观察测定离体大鼠正常肺泡巨噬细胞和博莱霉素A_6(BLM A_6)作用的肺泡巨噬细胞表面纤维粘连蛋白的荧光强度的变化以及活血化瘀类中药通脉灵单体764-3对上述处理细胞的影响。动物选用雄性Wista大鼠作为肺泡巨噬细胞供体,采用原位肺泡支气管灌流技术获得肺泡巨噬细胞并经贴壁进行纯化。用含10％小牛血清的DMEM培养液将细胞制成1×10~5/ml浓度,二氧化碳(卵鸟)箱培养24h后将细胞分4组。①746-3组,用终浓度为20μg/ml 764-3培养液作用1.5h。②BLM A_6+764-3组,764-3处理后再经终浓度为6ng/ml BLM A_6培养液作用2h。③BLM A_6组,用相同于2组剂量的BLM A_6作用
Indirect immunofluorescence assay was used to determine the changes of fluorescence intensity of fibronectin on alveolar macrophages in normal rat alveolar macrophages and bleomycin A_6 (BLM A_6) Effect of body 764-3 on the above treated cells. Animals were selected male Wistar rats as alveolar macrophages donor, alveolar macrophages were obtained by in situ alveolar-bronchial perfusion and purified by adherent. The cells were made into a concentration of 1 × 10 5 / ml in DMEM medium containing 10% fetal bovine serum, and the cells were divided into 4 groups after being cultured in a carbon dioxide (ovum) box for 24 hours. ①746-3 group, with a final concentration of 20μg / ml 764-3 culture medium for 1.5h. ② BLM A_6 + 764-3 group, 764-3 treated by the final concentration of 6ng / ml BLM A_6 medium for 2h. BLM A_6 group, with the same dose of two BLM A_6 role