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[目的]研究透明质酸介导纳米银靶向脂质体促进烧伤创面愈合作用,探讨透明质酸与磺胺嘧啶银的配比比例.[方法]制备透明质酸介导磺胺嘧啶银靶向脂质体,配方包括 100%磺胺嘧啶银(对照配方)、95%磺胺嘧啶银+5%透明质酸(配方A)、90%磺胺嘧啶银+10%透明质酸(配方B).建立深Ⅱ度烧伤SD大鼠模型54只,随机分为对照组、实验A组、实验B组,各18只,其烧伤创面分别涂覆对照配方、配方A、配方B药物,分别于涂药后 d7 、d14处死各组 6 只大鼠,测定其创面中羟脯氨酸、胶原比例和真皮细胞 DNA增殖周期变化;观测各组未处死 6 只大鼠创面愈合时间及残余创面面积变化.[结果]三组建模后 d7 创面组织羟脯氨酸含量、Ⅲ／Ⅰ型胶原比例均较建模后d3 升高,实验A组建模后d7 创面组织羟脯氨酸含量、Ⅲ／Ⅰ型胶原比例高于对照组同期水平,实验B组建模后 d7 创面组织羟脯氨酸含量、Ⅲ／Ⅰ型胶原比例高于实验 A组同期水平,差异有统计学意义(P ＜0.05).三组建模后 d5 、d7 、d9 、d11真皮细胞 G0／G1 期 DNA均较建模后 d3 减少, S期DNA均较建模后 d3 增加,差异有统计学意义(P ＜0.05).实验 A组建模后 d5 、d7 、d9 真皮细胞 G0／G1期DNA比例低于对照组,其建模后 d11真皮细胞 G0／G1 期DNA比例高于后者;实验 B组建模后 d5 、d7 真皮细胞 G0／G1 期DNA比例低于实验 A组,其建模后 d9 、d11真皮细胞 G0／G1 期 DNA比例高于后者,差异有统计学意义(P ＜0.05).三组建模后 d5 、d7 、d9 、d11残余创面面积均较建模后 d3 降低,实验 A组建模后 d5 、d7 、d9 、d11残余创面面积均低于对照组同期水平,实验B组建模后 d5 、d7 、d9 、d11残余创面面积均低于实验 A组同期水平,差异有统计学意义(P ＜0.05).对照组创面平均愈合时间为(14.46± 1.17)d,高于实验 A 组的(12.87±0.75)d,实验 A组创面平均愈合时间高于实验 B组的(11.87 ±0.55)d,差异有统计学意义(P ＜0.05).[结论]透明质酸介导纳米银靶向脂质体能够有效促进创面愈合,且 90%磺胺嘧啶与 10%透明质酸配比对于促进创面愈合的效果较为理想.“,”[Objective]To study the effect of hyaluronic acid (HA)mediated nano silver targeted liposomes on burn wound healing,and to explore the ratio of HA to Ag sulfadiazine.[Methods]The formulation includes 100% sulfadiazine silver (control formulation),95% sulfadiazine silver + 5% hyaluronic acid (formulation a),90% sulfadiazine silver+ 10% hyaluronic acid (formulation b).54 SD rats with deep second degree burn were randomly divided into control group,experimental group A and experimental group B,with 18 rats in each group.Six rats in each group were killed on d7 and d14 after the application of control formula,formula A and formula B respectively.The changes of hydroxyproline,collagen ratio and DNA proliferation cycle of dermal cells were measured.The time of wound healing and the area of residual wound were observed.[Results]The content of hydroxyproline and the proportion of typeⅢ／Ⅰ collagen in the wound tissue of group d7 were higher than that of group d3.The content of hydroxyproline and the proportion of typeⅢ／Ⅰ collagen in the wound tissue of group A were higher than that of group A.the content of hydroxyproline and the proportion of typeⅢ／Ⅰ collagen in the wound tissue of group B were higher than that of group A (P ＜0.05).The DNA in G0／G1 phase of dermal cells in d5 ,d7 ,d9 and d11 group was significantly lower than that in d3 group, and that in S phase was significantly higher than that in d3 group (P ＜0.05).The proportion of DNA in G0／G1 phase of d5 ,d7 and d9 dermal cells in the group A was lower than that in the control group,and the proportion of DNA in G0／G1 phase of d11 dermal cells in the group B was higher than that in the group A;the proportion of DNA in G0／G1 phase of d5 and d7 dermal cells in the group B was lower than that in the group A, and the proportion of DNA in G0／G1 phase of d9 and d11 dermal cells in the group B was higher than that in the group A (P ＜0.05).The area of residual wound in the group A was lower than that in the control group.The area of residual wound in the group B was lower than that in the group A (P ＜0.05).The average healing time of the control group was (14.46 ± 1.17)days,higher than that of the experimental group A (12.87 ± 0.75)days,and the average healing time of the experimental group A was higher than that of the experimental group B (11.87± 0.55 days),the difference was statistically significant (P ＜0.05).[Conclusion]Hyaluronic acid mediated nano silver targeting liposomes can effectively promote wound healing,and the combination of 90% sulfadiazine and 10% hyaluronic acid is ideal for promoting wound healing.