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橡胶微型插条体外培养的初期试验遇到了一些问题,如腋芽发芽率低,长出来的芽又难于生根。本研究的目的旨在解决这方面的问题。材料与方法试验材料是不知父本GT1、PB5/51、RRIM623无性系实生苗,在温室已栽培1—3年,从其茎干或根蘗取3—4厘米长带一个或几个腋芽的节段作外植体。外植体在漂白粉溶液中浸30分钟,再用蒸馏水冲洗3次。表
Initial trials of in vitro culture of rubber mini-inserts have encountered several problems, such as low germination rates of axillary buds and difficulty of buds growing out. The purpose of this study is to solve this problem. MATERIALS AND METHODS The test material was derived from the clones of male parent GT1, PB5 / 51 and RRIM623 clones, cultivated for 1-3 years in the greenhouse and 3-4 cm long stems or root tillers with one or several axillary buds Segments as explants. The explants are soaked in the bleach solution for 30 minutes and rinsed three times with distilled water. table