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以桃ＡＣＣ氧化酶基因组ＤＮＡ为探针，与伤处理诱导后桃叶片总ＲＮＡ进行点杂交，结果表明，未经伤处理的样品未能检测出杂交信号，伤处理后２－４ｈ左右信号达到最强，将其样品ｍＲＮＡ反转录成ｃＤＮＡ，通过反转录聚合酶链式反应（ＰＣＲ）得到一条０．９５ｋｂ的特异性扩增产物，该扩增产物能与桃ＡＣＣ氧化酶基因组探针杂交。将其克隆到加Ｔ载体上，筛选到重组克隆并进行酶谱分析表明其物理图谱与报道的完全一致；组建亚克隆并进行核苷酸序列测定，表明与桃ＡＣＣ氧化酶基因组ＤＮＡ的编码区核苷酸序列同源性为９９．８％。将该基因克隆到表达载体ｐＧＥＭＥＸ－１，在大肠杆菌中表达出６０ｋＤ左右大小的融合蛋白。
Using peach ACC oxidase genomic DNA as a probe, blot hybridization was performed with the total RNA of peach leaf after injury treatment. The results showed that the hybridization signal was not detected in the untreated sample, and the signal reached the peak after 2-4 h Strong, the sample mRNA reverse transcribed into cDNA by reverse transcription polymerase chain reaction (PCR) to obtain a 0.95kb specific amplification product, the amplification product with peach ACC oxidase genomic probe hybridization . The clones were cloned into T vector, and the clones were screened and analyzed by zymography. The physical map of the clones was identical with that reported. The subclones were constructed and the nucleotide sequences were determined. The results showed that the coding region of peach ACC oxidase genomic DNA Nucleotide sequence homology was 99.8%. The gene was cloned into the expression vector pGEMEX-1, and a fusion protein of about 60 kD was expressed in E. coli.