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[目的]观察吡格列酮(pioglitazone,PI)对高脂饮食大鼠心肌PPARγ-AP-1信号途径的影响及抗氧化作用。[方法]随机选取雄性SD大鼠24只,随机分为对照组(C)、高脂组(HF)、吡格列酮处理组(HF+PI)3组,每组8只。对照组饲喂基础饲料,其他两组饲喂高脂饲料。各组同时做灌胃处理分别饲以干预剂。于0周、3周、6周尾静脉采血测TG、TC、HDL-C含量及6周末脂质过氧化产物MDA含量及抗氧化SOD、GPx活性变化。6周末留取心肌组织。RT-PCR分析各组PPARγmRNA表达水平。Western blot检测心肌PPARγ、AP-1蛋白质水平。[结果](1)吡格列酮能降低高脂饮食大鼠血清TG、TC含量;(2)吡格列酮能显著降低血清MDA含量,增加血清SOD、全血GPx活性;(3)高脂饮食大鼠心肌PPARγmRNA表达水平明显降低;而吡格列酮能显著增加心肌PPARγmRNA表达水平;(4)高脂组心肌PPARγ蛋白表达水平降低,核转录因子AP-1呈高表达水平;而吡格列酮组心肌PPARγ蛋白表达水平较高脂组呈高水平表达,核转录因子AP-1表达水平较高脂组降低。[结论]吡格列酮能拮抗高脂饮食大鼠心肌PPARγ表达水平降低,并由此途径阻止心肌核转录因子AP-1表达水平的升高,并具有降血脂、抗氧化作用。
[Objective] To observe the effect of pioglitazone (PI) on myocardial PPARγ-AP-1 signal pathway and its anti-oxidation in high fat diet rats. [Method] Twenty - four male SD rats were randomly selected and randomly divided into control group (C), high fat group (HF) and pioglitazone group (HF + PI). The control group was fed the basal diet, while the other two groups were fed the high-fat diet. At the same time, each group was treated by gavage with interfering agents. The content of TG, TC and HDL-C in the tail vein at the 0th week, the 3rd week and the 6th week were measured, and the content of MDA and the activity of SOD and GPx in the 6th week were measured. 6 weeks to take myocardial tissue. RT-PCR analysis of each group PPARγmRNA expression levels. Western blot detection of myocardial PPARγ, AP-1 protein levels. [Results] (1) Pioglitazone could reduce the contents of TG and TC in serum of high-fat diet rats. (2) Pioglitazone could significantly decrease the content of serum MDA and increase the activity of GPx and GPx in serum. (3) PPARγmRNA While pioglitazone significantly increased the expression of PPARγmRNA in myocardium. (4) The expression of PPARγprotein in high-fat diet group was decreased and the expression of nuclear transcription factor AP-1 was high. The expression of PPARγprotein in pioglitazone group was higher than that in pioglitazone group Group showed high level of expression, nuclear transcription factor AP-1 expression levels lower than the high-fat group. [Conclusion] Pioglitazone can inhibit the expression of PPARγ in myocardium of high-fat diet and prevent the increase of myocardial AP-1 expression, and has the effects of lowering blood lipid and antioxidation.