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目的:观察正常气虚小鼠下丘脑-垂体-睾丸轴与睾酮合成调节相关基因的转录特征。方法:采用小鼠标准化诊法和辨证方法,筛选出正常气虚小鼠与正常小鼠,采用GeneChip Mouse Exon 1.0 ST Array等技术,检测其下丘脑-垂体-睾丸轴有关基因的转录水平。结果:与正常对照小鼠比较,正常气虚小鼠下丘脑Gnrh1表达略增;垂体上Gnrh受体上调,但Cga、Fshb和Lhb下调;睾丸Cyp11a1、Cyp17a1、Hsd3b1、Hsd3b6等与睾酮合成相关酶一致上调。结论:正常小鼠出现气虚证时,垂体促性腺激素表达量略减,而睾丸合成睾酮的系列关键酶的表达活跃,这构成了正常气虚证小鼠下丘脑-垂体-睾丸轴的特征。
Objective: To observe the transcriptional characteristics of hypothalamus-pituitary-testis axis and testosterone synthesis-related genes in normal Qi deficiency mice. Methods: Normal Qi deficiency mice and normal mice were screened out by mouse standardization method and syndrome differentiation method. GeneChip Mouse Exon 1.0 ST Array and other techniques were used to detect the transcriptional level of the hypothalamus-pituitary-testis axis related genes. Results: Compared with normal control mice, Gnrh1 expression was slightly increased in hypothalamus of normal Qi deficiency mice; Gnrh receptor was up-regulated in pituitary, but Cga, Fshb and Lhb were down-regulated; Cyp11a1, Cyp17a1, Hsd3b1 and Hsd3b6 in testis were consistent with testosterone- Increase. CONCLUSION: When qi deficiency syndrome occurs in normal mice, the expression of pituitary gonadotropin is slightly reduced, and the expression of key enzymes in testicular synthesis of testosterone is active, which constitutes the hypothalamus-pituitary-testicular axis in normal Qi deficiency syndrome mice.