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目的 对北京市某幼儿园集中发热疫情中的病例进行病因研究,分析其病原学特征.方法 采集该幼儿园13位发热患者的咽拭子标本,利用RT-PCR扩增肠道病毒VP1区基因序列,测序分析病原分子特征.结果 8名病例为肠道病毒核酸通用阳性,测序分析发现其中3名病例为CoxA4病原体阳性.其他5名病例为肠道病毒核酸通用引物扩增阴性.结论 引起此次集中发热疫情的病原体主要为肠道病毒CoxA4,分析VP1区序列它们为同一基因型病原,分别与中国北京2012年序列KJ818321、中国深圳2009年序列HQ728260,还有中国上海2010年序列KJ541164高度一致.“,”Objective To study the etiology of an aggregation fever event at a kindergarten in Beijing,then analyze the feature of the etiology.Methods 13 throat swab samples were collected from the fever children,and then VP1 regions were amplified by using reverse transcription-polymerase chain reaction methods,and the PCR products were sequenced.Results A total of 8 collected cases were enterovirus (EV) infection,and then 3 of them were identified as coxsackievirus A4 (CoxA4).Conclusions The etiology causing this aggregation fever events mainly was CoxA4,analyzing VP1 region sequences,found them were the same genotype pathogen all,and with Beijing,China 2012 sequence KJ818321,Shenzhen,China 2009 sequence HQ728260 and Shanghai,China 2010 sequences KJ541164 highly consistent.