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目的:建立小半夏加茯苓汤中总生物碱的含量测定方法。方法:用紫外分光光度法测定小半夏加茯苓汤中总生物碱的含量。以溴百里香草酚蓝为显色剂,λmax=414 nm,测得生物碱在2.2～7.7mg.L-1具有良好线性关系,平均回收率为97.38%,r=0.9998。结果:复方煎剂中总生物碱含量为0.05722%、拆方煎剂中总生物碱含量为0.02325%、单方煎剂中总生物碱含量为0.00847%。结论:该方法操作简便、准确、灵敏、重现性好,可用于小半夏加茯苓汤的质量控制。
Objective: To establish a method for the determination of total alkaloids in Xiaobanxia plus Poria Decoction. Methods: The content of total alkaloids in Xiaobanxia plus Poria Decoction was determined by UV spectrophotometry. With bromothymol blue as the color reagent, λmax = 414 nm, the alkaloids measured in 2.2 ~ 7.7mg.L-1 has a good linear relationship, the average recovery was 97.38%, r = 0.9998. Results: The total alkaloid content in compound decoction was 0.05722%, the total alkaloid content in decoction was 0.02325%, and the total alkaloid content in decoction was 0.00847%. Conclusion: The method is simple, accurate, sensitive and reproducible. It can be used for the quality control of Xiaobanxia plus Poria Decoction.