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腹水的实体瘤-肝细胞瘤27、沃科肉瘤、埃利希腹水癌均可分泌多核甙酸磷酸酶。此酶系一耐热,分子量为10,000左右的大分子糖蛋白类,它可促进杂交细胞的生长。将皮下接种沃科肉瘤的大鼠饲养9-10日,取出肿瘤,将肿瘤组织放在匀浆器中 (于1克肿瘤组织中加入25毫升缓冲剂)匀化。匀浆以15000g转速离心15分钟,取上在水浴上加热45分钟,用10,000g转速清离心10分钟,除去凝固蛋白,测定上清(实验标本Ⅰ)中多核甙酸磷酸酶的活性,其蛋白质含量5微克/毫升。另外,将热处理后的标本用干燥的(NH_4)_2·SO_4盐析,增强多核甙酸磷酸
Ascites solid tumor - hepatoma 27, Waco sarcoma, Ehrlich ascites carcinoma can secrete poly-nucleotide phosphatase. This enzyme is a heat-resistant, molecular weight of about 10,000 macromolecular glycoproteins, it can promote the growth of hybrid cells. Rats that were subcutaneously inoculated with Waco sarcoma were bred for 9 to 10 days. Tumors were removed and the tumor tissue was homogenized in a homogenizer (25 ml of buffer in 1 g of tumor tissue). The homogenate was centrifuged at 15,000 g for 15 minutes, taken on a water bath for 45 minutes, centrifuged at 10,000 g for 10 minutes to remove coagulation protein, and the activity of poly-nucleotidase in the supernatant (experimental specimen I) was measured. The protein Content of 5 micrograms / ml. In addition, the heat-treated specimens with salted (NH 4) 2 SO 4 salting out, enhanced poly nucleoside phosphate