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目的利用18F-脱氧葡萄糖(18F-FDG)细胞结合率早期评价放疗增敏剂效果。方法对胰腺癌Patu8988细胞单纯照射及加甘氨双唑钠(CMNa)和2-脱氧葡萄糖(2-DG)后进行照射,测定照射后24和48 h18F-FDG细胞结合率。结果 24 h后,单纯照射组各照射剂量组间的18F-FDG细胞结合率差异均无统计学意义(均P>0.05);2-DG组和CMNa组各照射剂量组的18F-FDG细胞结合率差异均有统计学意义,且均明显低于单纯照射组(P<0.05或<0.01)。48 h后,各组18F-FDG细胞结合率随照射剂量的增加而明显降低(均P<0.05);2-DG组和CMNa组各照射剂量组的18F-FDG细胞结合率显著低于单纯照射组(均P<0.01)。24和48 h后,MTT测定吸光度(A)值与18F-FDG细胞结合率成正相关(r=0.759,r=0.814,均P<0.01)。结论 2-DG或CMNa联合照射后,24 h可引起胰腺癌Patu 8988细胞18F-FDG细胞结合率下降,提示可用18F-FDG显像早期观测放疗对胰腺癌的辐射效应。
OBJECTIVE: To evaluate the effect of radiosensitizer with 18F-deoxyglucose (18F-FDG) cell binding rate. Methods Patu8988 cells were irradiated with CMNa and 2-deoxyglucose (2-DG) after irradiation. The binding rate of 18F-FDG cells was measured at 24 and 48 h after irradiation. Results After 24 h, there was no significant difference in the 18F-FDG cell binding rate between the two groups (all P> 0.05). The number of 18F-FDG cells in the 2-DG group and the CMNa group was significantly decreased The differences were statistically significant (P <0.05 or <0.01). After 48 h, the binding rate of 18F-FDG cells in each group decreased significantly with the increase of irradiation dose (all P <0.05); the binding rate of 18F-FDG cells in 2-DG group and CMNa group was significantly lower than that of simple irradiation Group (all P <0.01). After 24 and 48 h, the absorbance (A) value of MTT assay was positively correlated with the binding rate of 18F-FDG cells (r = 0.759, r = 0.814, all P <0.01). Conclusions The combination of 2-DG or CMNa irradiation, 24 h can cause pancreatic cancer Patu 8988 cells 18F-FDG cell binding decreased, suggesting that 18F-FDG imaging early observation of radiation effects of radiation on pancreatic cancer.