论文部分内容阅读
为探讨箭舌豌豆根响应镉胁迫的分子机理,以两个镉耐性差异的箭舌豌豆品种(镉耐性品种‘L3’,镉敏感品种‘ZM’)为材料,通过双向电泳和基质辅助激光解析/电离时间飞行质谱技术以及NCBI数据库搜索,分析25μmol·L~(-1) CdCl_2处理5 d后两个品种根蛋白质的差异表达。结果表明,镉胁迫下‘L3’和‘ZM’根共有56个蛋白点的表达量发生显著变化,其中5个蛋白点在两个品种根中共同上调,1个蛋白点共同下调,4个蛋白点为镉处理后两个品种中共同新出现。28个差异表达蛋白点得到鉴定,这些蛋白质的功能可分为七类:(1)抗氧化与解毒相关蛋白;(2)呼吸与能量代谢相关蛋白;(3)胁迫响应相关蛋白;(4)信号转导相关蛋白;(5)细胞壁合成相关蛋白;(6)细胞增殖相关蛋白;(7)未知功能蛋白。镉胁迫下耐性品种‘L3’较敏感品种‘ZM’鉴定到更多抗氧化解毒、胁迫响应和细胞增殖蛋白的上调表达,这可能是耐性品种‘L3’较敏感品种‘ZM’具有更强镉耐性的重要原因;生长素、水杨酸和乙烯诱导的信号转导途径可能参与箭舌豌豆对镉胁迫的响应。
In order to explore the molecular mechanism of response to cadmium stress, the two pea cultivars (Cd tolerant cultivar ’L3’ and Cd-sensitive cultivars ’ZM’) with different cadmium tolerance were used as experimental materials. / Ionization time-of-flight mass spectrometry (GC-MS / MS) and NCBI database, the differential expression of root proteins in two cultivars was analyzed after treatment with 25μmol·L -1 CdCl 2 for 5 days. The results showed that 56 protein spots of ’L3’ and ’ZM’ roots changed significantly under cadmium stress. Among them, five protein spots were up-regulated in two varieties of roots, one was down-regulated, four were Point of cadmium treatment after two new co-emergence. 28 differentially expressed protein spots were identified. The function of these proteins can be divided into seven categories: (1) antioxidative and detoxification related proteins; (2) respiratory and energy metabolism related proteins; (3) stress response related proteins; (4) Signal transduction related proteins; (5) Cell wall synthesis related proteins; (6) Cell proliferation related proteins; (7) Unknown functional proteins. The results showed that more resistant and detoxification, stress response and up-regulated expression of cell proliferation proteins were identified in ’ZM’, a tolerant cultivar ’L3’ under Cd stress. This may be due to the stronger tolerance of ’ZM’ The main reason of tolerance is that the signal transduction pathways induced by auxin, salicylic acid and ethylene may be involved in the response of A. tabaci to cadmium stress.