【摘 要】
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Inhibitory GABAergic intemeurons are fundamental elements of cortical circuits and play critical roles in shaping network activity.Dysfunction of interneurons can lead to various brain disorders,including epilepsy,schizophrenia,and anxiety.Based on the el
【机 构】
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College of Pharmaceutical Sciences, Brain Institute, Capital Medical University, Beijing 100069, Chi
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Inhibitory GABAergic intemeurons are fundamental elements of cortical circuits and play critical roles in shaping network activity.Dysfunction of interneurons can lead to various brain disorders,including epilepsy,schizophrenia,and anxiety.Based on the electrophysiological properties,cell morphology,and molecular identity,interneurons could be classified into various subgroups.In this study,we investigated the density and laminar distribution of different interneuron types and the coexpression of molecular markers in epileptic human cortex.We found that parvalbumin (PV) and somatostatin (SST)neurons were distributed in all cortical layers except layer Ⅰ,while tyrosine hydroxylase (TH) and neuropeptide Y (NPY) were abundant in the deep layers and white matter.Cholecystokinin (CCK) neurons showed a high density in layers Ⅳ and Ⅵ.Neurons with these markers constituted ～7.2％ (PV),2.6％ (SST),0.5％ (TH),0.5％ (NPY),and 4.4％ (CCK) of the gray-matter neuron population.Doubleand triple-labeling revealed that NPY neurons were also SST-immunoreactive (97.7％),and TH neurons were more likely to express SST (34.2％) than PV (14.6％).A subpopulation of CCK neurons (28.0％) also expressed PV,but none contained SST.Together,these results revealed the density and distribution patterns of different interneuron populations and the overlap between molecular markers in epileptic human cortex.
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