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AIM:To investigate whether IL-10-transduced dendritic cells(DCs) could induce tolerogenicity and prolong allograftsurvival in rat intestinal transplantation.METHODS:Spleen-derived DCs were prepared and geneticallymodified by hIL-10 gene.The level of IL-10 expression wasquantitated by ELISA.DC function was assessed by MTT inmixed leukocyte reaction.Allogeneic T-cell apoptosis wasexamined by flow cytometric analysis.Seven days beforeheterotopic intestinal transplantation,2×10~6 donor-derivedIL-10-DC were injected intravenously,then transplantationwas performed between SD donor and Wistar recipient.RESULTS:Compared with untransduced DC,IL-10-DC couldsuppress allogeneic mixed leukocyte reaction (MLR).Theinhibitory effect was the most striking with the stimulator/effector (S/E) ratio of 1:10.The inhibition rate was 33.25 %,41.19 % (P<0.01) and 22.92 % with the S/E ratio of 1:1,1:10 and 1:50 respectively.At 48 hours and 72 hours byflow cytometry counting,apoptotic T cells responded to IL-10-DC in MLR were 13.8 % and 30.1%,while untransducedgroup did not undergo significant apoptosis (P<0.05).IL-10-DC pretreated recipients had a moderate survival prolongationwith a mean allograft survival of 19.8 days (P<0.01),compared with 7.3±2.4 days in control group and 8.3±2.9days in untransduced DC group.Rejection occurred in thecontrol group within three days.The difference betweenuntreated DC group and control group was not significant.CONCLUSION:IL-10-DC can induce allogenic T-cellhyporesponsiveness in vitro and apoptosis may be involvedin it.IL-10-DC pretreatment can prolong intestinal allograftsurvival in the recipient.
AIM: To investigate whether IL-10-transduced dendritic cells (DCs) could induce tolerogenic and prolong allograftsurvival in rat intestinal transplantation. METHODS: Spleen-derived DCs were prepared and genetically modified by hIL-10 gene. The level of IL-10 expression wasquantitated by ELISA. DC function was assessed by MTT in mixed leukocyte reaction. Allogeneic T-cell apoptosis wasexamined by flow cytometric analysis. Seven days before hepototopic intestinal transplantation, 2 × 10 -6 donor-derived IL-10-DC were injected intravenously, then transplantation was performed between between SD donor and Wistar recipient .RESULTS: Compared with untransduced DC, IL-10-DC couldsuppress allogeneic mixed leukocyte reaction (MLR). The inhibitory effect was the most striking with the stimulator / effector (S / E) ratio of 1: 10.The inhibition rate was 33.25%, 41.19% (P <0.01) and 22.92% with the S / E ratio of 1: 1,1: 10 and 1:50 respectively. At 48 hours and 72 hours byflow cytometry counting, apoptotic T cells responded to IL-10 -DC in MLR were 13.8% and 30.1%, while untransducedgroup did not undergo significant apoptosis (P <0.05). IL-10-DC pretreated recipients had a moderate survival prolongation with a mean allograft survival of 19.8 days (P <0.01) with 7.3 ± 2.4 days in control group and 8.3 ± 2.9 days in untransduced DC group. Rejection occurred in the control group within three days. The difference betweenunt DC group and control group was not significant. CONCLUSION: IL-10-DC can induce allogenic T -cellhyporesponsiveness in vitro and apoptosis may be involved in it. IL-10-DC pretreatment can prolong intestinal allograftsurvival in the recipient.