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目的 :利用子宫胎盘缺血术诱发大鼠妊娠期高血压,测定妊娠期高血压大鼠血浆中生长分化因子(growth differentiafion factor-15,GDF-15)表达水平。方法:清洁级健康雌性Spragu-Dawley大鼠20只,随机分成2组,每组10只,分为假手术对照组及子宫胎盘灌注下降(reduced uterine perfusion pressure,RUPP)手术组。所有大鼠自然受孕,在妊娠期14 d对妊娠大鼠进行手术,在妊娠期13 d以及19 d分别测定大鼠血压。于妊娠19 d采血测定大鼠血浆GDF-15含量。结果:RUPP手术组妊娠19 d收缩压、舒张压与妊娠13 d相比均显著升高[(157.8±9.0)mmHg vs(118.3±5.7)mmHg;(122.5±6.9)mmHg vs(88.4±8.0)mmHg,P<0.05],假手术组则升高不明显。妊娠19 d RUPP手术组与假手术组相比,收缩压、舒张压均显著升高[(157.8±9.0)mmHg vs(117.0±9.4)mmHg;(122.5±6.9)mmHg vs(100.7±4.4)mmHg,P<0.05]。与假手术组相比,RUPP手术组妊娠19 d血浆中GDF-15浓度明显增高[(363.3±78.1)ng/L vs(276.7±69.4)ng/L,P<0.05)。结论:妊娠期高血压大鼠动物模型血浆中GDF-15表达水平明显增高,GDF-15是一个预测妊娠期高血压患者预后的潜在指标。
OBJECTIVE: To detect the gestational hypertension in rats induced by uterine placental ischemia, and to determine the expression of GDF-15 in plasma of hypertensive rats during pregnancy. Methods: Twenty healthy female Spragu-Dawley rats were randomly divided into 2 groups (10 rats in each group), which were divided into sham-operation control group and reduced uterine perfusion pressure (RUPP) operation group. All rats were spontaneously pregnant, pregnant rats were operated on the 14th day of gestation, and blood pressure was measured on the 13th and 19th day of gestation. Plasma levels of GDF-15 were measured at 19 d of gestation. Results The systolic blood pressure and diastolic blood pressure were significantly increased at 19 d of gestation in RUPP group [(157.8 ± 9.0) mmHg vs (118.3 ± 5.7) mmHg; (122.5 ± 6.9) mmHg vs (88.4 ± 8.0) mmHg, P <0.05], while the sham-operated group showed no obvious increase. Systolic and diastolic blood pressure were significantly higher in RUPP surgery group than in sham operation group on the 19th day of gestation (157.8 ± 9.0 mmHg vs 117.0 ± 9.4 mmHg, 122.5 ± 6.9 mmHg vs 100.7 ± 4.4 mmHg , P <0.05]. Compared with the sham operation group, the GDF-15 concentration in the plasma increased significantly at 19 d after gestation in the RUPP group [(363.3 ± 78.1) ng / L vs (276.7 ± 69.4) ng / L, P <0.05). Conclusion: The expression of GDF-15 in the plasma of pregnancy-induced hypertensive rats was significantly increased. GDF-15 is a potential indicator of the prognosis of patients with gestational hypertension.