论文部分内容阅读
目的建立同时检测沙门菌和金黄色葡萄球菌的二重PCR方法。方法本试验根据沙门菌inv A基因序列及金黄色葡萄球菌nuc基因序列设计引物,建立二重PCR方法,并采用该方法进行核酸扩增,再将已建立的二重PCR方法应用到肉鸡养殖场中。结果经扩增后,检出沙门菌284 bp及金黄色葡萄球菌484 bp的特异性扩增条带,而其他普通的致病菌未检测出来,二重PCR能在一个反应体系中同时检测出沙门菌及金黄色葡萄球菌。对1 231份肉鸡粪便样品检测发现,沙门菌阳性40份,阳性率为2.33%,金黄色葡萄球菌阳性101份,阳性率为8.22%。结论建立的沙门菌及金黄色葡萄球菌二重PCR方法,检测的敏感性高、特异性较强,能提高临床粪便致病菌检测的效率及准确率。
Objective To establish a duplex PCR method for the simultaneous detection of Salmonella and Staphylococcus aureus. Methods Based on the sequence of inv A gene of Salmonella and the sequence of nuc gene of Staphylococcus aureus, a duplex PCR method was designed and used for nucleic acid amplification. The established duplex PCR method was applied to broiler farms in. Results After amplification, the specific amplified bands of 284 bp of Salmonella and 484 bp of Staphylococcus aureus were detected, while other common pathogens were not detected. Duplex PCR was able to detect simultaneously in one reaction system Salmonella and Staphylococcus aureus. A total of 1 231 samples of broiler stool samples were detected, and 40 were positive for Salmonella, the positive rate was 2.33%. The positive rate of Staphylococcus aureus was 101, the positive rate was 8.22%. Conclusion The established multiplex PCR method of Salmonella and Staphylococcus aureus has high sensitivity and specificity, which can improve the efficiency and accuracy of detection of clinical stool pathogens.