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1 材料与方法11 病料来源患病异育银鲫来自南京某部队养殖场。12 细菌分离鉴定选取症状典型、濒临死亡的病鱼,清水冲洗后,用75%的酒精对病鱼体表进行擦拭消毒,按常规方法,无菌取病鱼腹水、肝、肾,接种于普通营养琼脂平板上,28℃培养,24ｈ观察结果。病原菌生物学性状的测定按
1 Materials and Methods 11 sources of disease-borne heterophylla crucian carp from a military farm in Nanjing. 12 bacterial isolation and identification Select the typical symptoms of the moribund fish disease, rinse with water, with 75% alcohol wipe the diseased fish body surface disinfection, according to conventional methods, aseptic diseased fish ascites, liver, kidney, inoculated in ordinary Nutritional agar plate, 28 ℃ culture, 24h observation results. Determination of biological characteristics of pathogens press