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目的探讨原发性肝癌(PHC)患者血清miR-1的表达及其与临床病理特征之间的关系。方法采用实时定量PCR检测90例PHC患者及102例健康志愿者血清中miR-1的表达量。结果 PHC组中miR-1表达量[0.210(0.127~0.312)]明显低于健康对照组[0.780(0.618~1.121)]及PHC术后7 d组[0.540(0.318~0.716)](P<0.001)。在90例PHC患者中,miR-1表达水平与淋巴结有无转移密切相关,有淋巴结转移患者中miR-1表达水平显著低于无淋巴结转移患者(P=0.003)。Kaplan-Meier分析显示miR-1低表达组的生存率远低于其高表达组,其中位生存期(月)分别为25.48和40.98(P<0.001)。Cox多变量分析显示miR-1水平是影响PHC患者生存率的独立危险因素之一,相对危险度为0.424(95%CI:0.183~0.984)。结论血清miR-1作为新的生物学指标对于PHC的诊断和预后的具有一定的价值。
Objective To investigate the expression of miR-1 in patients with primary liver cancer (PHC) and its relationship with clinicopathological features. Methods Real-time quantitative PCR was used to detect the expression of miR-1 in 90 PHC patients and 102 healthy volunteers. Results The expression level of miR-1 in PHC group [0.210 (0.127-0.312)] was significantly lower than that in healthy control group [0.780 (0.618-1.121)] and PHC group [0.540 (0.318-0.716) ). In 90 PHC patients, miR-1 expression was closely related to lymph node metastasis, and miR-1 expression in patients with lymph node metastasis was significantly lower than that in patients without lymph node metastasis (P = 0.003). Kaplan-Meier analysis showed that the survival rate of miR-1 low expression group was much lower than that of its high expression group (median survival was 25.48 and 40.98, respectively; P <0.001). Cox multivariate analysis showed that miR-1 level was an independent risk factor affecting the survival rate of patients with PHC. The relative risk was 0.424 (95% CI: 0.183-0.984). Conclusion Serum miR-1 as a new biological index has certain value for the diagnosis and prognosis of PHC.