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目的 :探讨熊脱氧胆酸 (U DCA)和鹅脱氧胆酸 (CDCA)对胆汁分泌、谷氨酸脱氢酶 (GL DH)和线粒体膜结构的作用 ,以及 U DCA 的线粒体膜保护作用。 方法 :应用离体大鼠肝灌注技术 ,0 .1～ 0 .5 mm ol/ L 胆酸灌注鼠肝 ,测定胆汁分泌及GL DH释放。分离鼠肝线粒体 ,自旋标记物插入线粒体膜 ,研究膜结构变化。结果 :CDCA(0 .1、0 .3、0 .5 m mol/ L)可使胆汁分泌分别减少 12 %、77.2 5 %和 78.98% ,明显增加 GL DH释放达 3、9和 12倍 ,并增加线粒体膜的流动性 ;UDCA在 0 .3、0 .5mm ol/ L 时可增加胆汁分泌达 1.8倍和 1.9倍 ,不影响 GL DH和线粒体膜结构。预灌注 UDCA可部分缓解 CDCA引起的GL DH释放和胆汁分泌减少 ,稳定线粒体膜结构。结论 :CDCA破坏线粒体膜和引起肝功能减退。U DCA可改善胆汁分泌 ,部分阻止 CDCA对线粒体的损害。低浓度 CDCA不损害肝功能。
AIM: To investigate the effects of UDCA and CDCA on bile secretion, glutamate dehydrogenase (GL DH) and mitochondrial membrane structure, and mitochondrial membrane protection of U DCA. Methods: Isolated rat liver perfusion technique was used to perfuse rat liver with 0.5-1.5 mmol / L cholic acid to determine bile secretion and GL DH release. Isolated rat liver mitochondria, spin markers inserted into the mitochondrial membrane, membrane structure changes. Results: CDCA (0.1, 0.3, 0.05 mol / L) reduced bile secretion by 12%, 77.2% and 78.98%, respectively, and significantly increased GL DH release by 3, 9 and 12 times Increased the fluidity of mitochondrial membrane; UDCA increased bile secretion by 1.8 times and 1.9 times at 0.3, 0.5mm ol / L, which did not affect GL DH and mitochondrial membrane structure. Pre-infusion of UDCA partially mitigated CDCA-induced GL DH release and decreased bile secretion, stabilizing the mitochondrial membrane structure. Conclusion: CDCA destroys the mitochondrial membrane and causes hepatic dysfunction. U DCA improves bile secretion and partially prevents CDCA damage to mitochondria. Low concentrations of CDCA do not impair liver function.