中国原产果梅自交亲和变异品种花粉决定基因SFB的插入突变

• 作 者: 王培培 侍婷 高志红 章镇 庄维兵
• 机 构: 南京农业大学园艺学院,
• 出 处: 园艺学报
• 发表日期: 2012年03期
• 关键词: 自交亲和 SFB 果梅 自交亲和性 花粉 AS-PCR 长农 自交不亲和 自花授粉 插入突变

果梅是配子体自交不亲和树种,但在长期进化过程中也出现了自交亲和品种。以果梅典型的自交不亲和品种‘南高’和自交亲和品种‘甲州小梅’为对照,采用田间自交授粉试验对原产于中国的‘四川白梅’和‘长农17’两个果梅品种进行了自交亲和性鉴定,结果表明:其自花授粉坐果率分别为30.73%和16.27%,属于自交亲和品种。进一步通过AS-PCR分析发现花粉SFB基因存在一段插入序列,造成了分子水平的基因突变。推测该基因的突变使‘四川白梅’和‘长农17’SFB功能发生改变,从而自交亲和。

Fruit plum is self-incompatible gametophyte species, but in the long-term evolution has also emerged self-compatible varieties. Taking ’South High’, a typical self-incompatible variety of plum, and self-compatible ’Xiaomei,’ as control, self-pollination experiments were conducted in the field of ’Sichuan Baimei’ and ’Longnong 17’ The results showed that the self-pollination and fruit-setting rate were 30.73% and 16.27% respectively, belonging to the self-compatible varieties. Further analysis of the AS-PCR revealed that there was an inserted sequence in the pollen SFB gene, resulting in a molecular mutation at the gene level. It is speculated that the mutation of this gene causes the function of ’Sichuan white plum’ and ’Changnong 17’SFB to be changed, thus self-compatible.