Overexpression of rice OsLOL2 gene confers disease resistance in tobacco to Pseudomonas syringae pv.

• 作 者: Khizar Hayat Bhatti
• 机 构: State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences,,Beiji
• 出 处: Progress in Natural Science
• 发表日期: 2008年07期
• 关键词: tobacco tabaci Arabidopsis transgenic hypersensitive finger cloned bacterium dis

LSD1-related proteins of Arabidopsis with LSD1-like zinc finger domains regulate disease resistance and programmed cell death (PCD). We cloned a rice OsLOL2 gene, orthologous to LSD1 of Arabidopsis and expressed it in a tobacco plant. Transgenic tobacco lines displayed enhanced disease resistance to a virulent bacterium Pseudomonas syringae pv. tabaci (Pst). RT-PCR analysis showed that overexpression of OsLOL2 in transgenic tobacco lines resulted in upregulation of two pathogenesis-related (PR) protein genes, PR2 and PR5. Our results suggest that overexpression of OsLOL2 in transgenic tobacco enhances the resistance through the induction of PR pro- teins and hypersensitive response-like reaction.

LSD1-related proteins of Arabidopsis with LSD1-like zinc finger domains regulate disease resistance and programmed cell death (PCD). We cloned a rice OsLOL2 gene, orthologous to LSD1 of Arabidopsis and expressed it in a tobacco plant. resistance to a virulent bacterium Pseudomonas syringae pv. tabaci (Pst). RT-PCR analysis showed that overexpression of OsLOL2 in transgenic tobacco lines resulted in upregulation of two pathogenesis-related (PR) protein genes, PR2 and PR5. Our results suggest that overexpression of OsLOL2 in transgenic tobacco enhances the resistance through the induction of PR pro- teins and hypersensitive response-like reactions.