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目的:观察Ⅰ型NKT细胞在哮喘小鼠气道炎症形成中作用。方法:24只BALB/c小鼠随机分为对照组、哮喘组和过继转移组,每组8只;随机选取8只CD1d-/--BALB/c小鼠为CD1d-/-组。对照组和哮喘组、CD1d-/-组分别采用PBS和卵清白蛋白(OVA)致敏和激发,过继转移组采用OVA致敏,在第1次激发前24h给予Ⅰ型NKT细胞尾静脉注射。分别采用HE和PAS染色检测肺组织学和气道杯状细胞;姬姆萨染色检测支气管肺泡灌洗液(BALF)各细胞分类计数;ELISA法检测血清OVA特异性IgE和IgG1及BALF中IL-4、IL-5和IL-13水平;流式细胞仪检测肺Ⅰ型NKT细胞、IL-4+和IFN-γ+Ⅰ型NKT细胞的数量。结果:哮喘组小鼠肺Ⅰ型NKT细胞、IL-4+和IFN-γ+Ⅰ型NKT细胞的数量明显高于对照组(均P<0.05);过继转移组小鼠肺组织炎性细胞和气道基底膜杯状细胞增多,过继转移组小鼠BALF中嗜酸细胞数量、血清OVA特异性IgE和IgG1及BALF中IL-4、IL-5和IL-13水平明显高于哮喘组(均P<0.05);CD1d-/-组小鼠肺组织炎性细胞和气道基底膜杯状细胞增多,CD1d-/-组小鼠BALF中嗜酸细胞数量、血清OVA特异性IgE和IgG1及BALF中IL-4、IL-5和IL-13水平明显低于哮喘组,但明显高于对照组(均P<0.05)。结论:Ⅰ型NKT细胞可以增强哮喘小鼠气道炎症,但并不是哮喘小鼠气道炎症形成的必需因素。
Objective: To observe the role of type Ⅰ NKT cells in the formation of airway inflammation in asthmatic mice. Methods: 24 BALB / c mice were randomly divided into control group, asthma group and adoptive transfer group, with 8 mice in each group. Eight CD1d - / - BALB / c mice were randomly selected as CD1d - / - group. The control group and the asthma group were sensitized and challenged with PBS and ovalbumin (OVA) respectively. The adoptive transfer group was sensitized with OVA, and the type Ⅰ NKT cells were injected into tail vein 24 hours before the first challenge. Lung histology and airway goblet cells were detected by HE staining and PAS staining respectively. The counts of bronchoalveolar lavage fluid (BALF) were detected by Giemsa staining. Serum OVA-specific IgE and IgG1 and IL-4 , IL-5 and IL-13 levels. The numbers of type I NKT cells, IL-4 + and IFN-γ + type I NKT cells were detected by flow cytometry. Results: The numbers of type Ⅰ NKT cells, IL-4 + and IFN-γ + type Ⅰ NKT cells in asthmatic mice were significantly higher than those in control group (all P <0.05). In adoptive transfer group, the number of inflammatory cells and gas The numbers of goblet cells in BALF, the level of serum OVA-specific IgE and IgG1 and the levels of IL-4, IL-5 and IL-13 in BALF of adoptive transfer group were significantly higher than those of asthma group <0.05). The number of inflammatory cells and airway basement membrane goblet cells in CD1d - / - mice increased, the numbers of eosinophils in BALF, the levels of serum specific IgE and IgG1 in BALF and IL- -4, IL-5 and IL-13 levels in asthma group were significantly lower than those in control group (all P <0.05). CONCLUSION: Type I NKT cells can enhance airway inflammation in asthmatic mice, but they are not essential for airway inflammation in asthmatic mice.