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为探究苜蓿(Medicago sativa L.)和扁蓿豆(Medicago ruthenica(L.)Trautv.)种子萌发期的耐盐性强弱,采用复盐溶液模拟盐胁迫的方法,研究了不同浓度梯度(0.3%、0.6%、0.9%、1.2%、1.5%)盐胁迫对5份野生苜蓿和5份野生扁蓿豆种子萌发的影响。测定了发芽率、发芽势、发芽指数、活力指数、根长、苗长、幼苗干重,利用方差分析、相关分析、主成分分析、隶属函数分析和灰色关联分析进行了耐盐性综合评价。结果表明相同指标不同浓度盐溶液和不同材料之间多存在显著差异,各指标之间存在不同程度的相关关系。各指标中与耐盐强弱最相关的指标为幼苗干重,其关联度为0.752,其次为发芽率,关联度为0.745。供试10份材料中来源于中国内蒙古通辽市大青沟自然保护区的扁蓿豆和中国内蒙古呼和浩特武川县的扁蓿豆耐盐性较强,其综合耐盐D值和关联度均较高,分别为0.807、0.699和0.793、0.716。总体比较供试苜蓿和扁蓿豆材料,扁蓿豆萌发期耐盐性较强。
In order to explore the salt-tolerance of Medicago sativa L. and Medicago ruthenica (L.) Trautv. During seed germination, the effects of different salt concentrations (0.3 %, 0.6%, 0.9%, 1.2%, 1.5%) on the germination of five wild alfalfa and five wild alfalfa seeds under salt stress. Germination rate, germination potential, germination index, vigor index, root length, seedling length and seedling dry weight were determined. The comprehensive evaluation of salt tolerance was carried out by using variance analysis, correlation analysis, principal component analysis, membership function analysis and gray relational analysis. The results showed that there were significant differences between the different concentrations of salt solution and different materials of the same index, and there were some correlations between the indexes. The most relevant indicator of salt tolerance was the dry weight of seedlings, the correlation degree was 0.752, followed by the germination rate, the correlation degree was 0.745. Among the 10 materials tested, the alfalfa beans derived from Daqinggou Nature Reserve in Tongliao City, Inner Mongolia, China, and the alfalfa beans in Wuchuan County, Huhehot, Inner Mongolia, China had a higher salt tolerance and a higher integrated salt tolerance D value and correlation degree , Respectively, 0.807,0.699 and 0.793,0.716. The overall comparison of alfalfa and Medicago truncatula materials, Medicago sativa germination phase salt tolerance.