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研究并确定了醋酸氯已定与甲酚红的荷移反应条件。在乙醇-丙酮介质中形成1：1型络合物，其λmax=455nm，醋酸氯已定浓度在0～80mg/L范围内呈线性关系；表观摩尔吸光系数为1．08×104L·mol-1·cm-1；桑德尔灵敏度是0．0345μg·cm-2；相对标准偏差小于1％（n＝8），回收率符合要求。
The charge-transfer reaction conditions of chlorhexidine acetate and cresol red were studied and determined. In the ethanol-acetone medium, the 1: 1 complex was formed, the λmax = 455nm, the linear range of the concentration of chloroacetic acid was 0 ~ 80mg / L, the apparent molar absorptivity was 1.08 × 104L · mol -1 · cm-1; Sensitivity of Sander was 0.0345μg · cm-2; the relative standard deviation was less than 1% (n = 8). The recovery was satisfactory.