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以小麦幼穗和幼胚为外植体，诱导出愈伤组织，通过在培养基中逐渐增加选择剂（ＮａＣｌ：Ｎａ２ＳＯ４＝４：１）方法，获得耐０．８％至２．０％选择剂的耐盐愈伤组织，其内源脯氨酸含量显著高于相同盐渍条件下的对照组．培养基中选择剂浓度达到１．６％耐盐细胞仍能保持细胞及细胞器正常形态，液泡化程度低，细胞核大，有的细胞中出现二或二个以上核仁．在培养基中选择剂超过１．２％，绝大多数细胞处于坏死或类坏死状态．耐盐愈伤组织在培养基中选择剂不超过２．０％，生长良好，可以再生植株，其后代在０．３％ＮａＣｌ土壤中仍表现耐盐性．
Callus was induced by using young ears and young embryos as explants, and 0.8% to 2.0% selection was obtained by gradually increasing the selection agent (NaCl: Na2SO4 = 4: 1) in the medium Salt tolerant callus, the endogenous proline content was significantly higher than the control group under the same saline conditions. The selection of the medium concentration of 1.6% salt-tolerant cells can still maintain the normal morphology of cells and organelles, the degree of vacuolization, large nuclei, and some cells appear two or more nucleoli. In the medium more than 1.2% of the selection agent, the vast majority of cells in necrosis or necrosis. Salt-tolerant callus did not exceed 2.0% of the selective agent in the medium, grew well and regenerated the plants, and its progenies still showed salt tolerance in 0.3% NaCl soil.