Alternative splicing of the FMR1 gene in human tissues of fetal and adult

• 作 者: 丁侃 李兰英 范钰 许琪 韩富天 朱宁宁 刘恩莉 林炳承 吴冠芸 沈岩
• 机 构: State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union
• 出 处: Progress in Natural Science
• 发表日期: 1999年05期
• 关键词: splicing fetal fetus Seven developmental capillary switch abundance brainstem sp

To study the time- and tissue-specificity of alternative splicing of the FMRl gene, we analyzed the alternative splicing pattern of the FMRl gene in human tissues from adult and fetus using RT-PCR coupled with capillary electrophoresis. Seven alternative splicing variants of FMRl were found in adult liver and lung. The major three alternative splicing variants of the FMRl gene in all analyzed fetal tissues were same, though the number of minor iso-forms and the relative abundance of major isoforms were different. The major difference of the alternative splicing pattern between adult and fetus was in exon 12 and 17. The results suggest that the alternative splicing pattern of the FMR1 gene is non-tissue-specific in the same developmental stage and a developmental switch may be present.

To study the time- and tissue-specificity of alternative splicing of the FMRl gene, we analyzed the alternative splicing pattern of the FMRl gene in human tissues from adult and fetus using RT-PCR coupled with capillary electrophoresis. Seven alternative splicing variants of FMRl were found in adult liver and lung. The major three alternative splicing variants of the FMRl gene in all analyzed fetal tissues were same, though the number of minor iso-forms and the relative abundance of major isoforms were different. The major difference of the alternative splicing pattern between adult and fetus was in exon 12 and 17. The results suggest that the alternative splicing pattern of the FMR1 gene is non-tissue-specific in the same developmental stage and a developmental switch may be present.