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目的 :探讨水通道蛋白4(AQP4)在急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠脑组织中的变化趋势,观察其与血脑屏障通透性和脑水肿的相关性。方法:84只成年雄性SD大鼠,随机分为ANP组(n=72)和假手术组(n=12)。制备ANP大鼠模型。假手术组于造模后24 h,ANP组于注射牛磺胆酸钠造模后3、6、9、12、18和24h 6个时间点为6个组,检测脑组织含水量、血脑屏障通透性变化,Western印迹法和半定量逆转录PCR检测脑组织AQP4蛋白及m RNA表达。结果 :脑组织AQP4蛋白及m RNA在损伤后6 h开始表达上调,9、12、18和24 h依次升高(P<0.05)。AQP4蛋白及m RNA的表达与脑组织含水量均呈正相关(P<0.05)。结论:ANP大鼠的血脑屏障功能降低及脑组织AQP4蛋白及m RNA表达水平增加,可能与脑水肿的形成和发展密切相关。
Objective: To investigate the change trend of aquaporin 4 (AQP4) in brain tissue of rats with acute necrotizing pancreatitis (ANP) and to observe its correlation with permeability of blood-brain barrier and cerebral edema. Methods: Eighty-four adult male Sprague Dawley rats were randomly divided into ANP group (n = 72) and sham operation group (n = 12). ANP rat model was prepared. Sham operation group at 24 h after modeling, ANP group at 6, 6, 9, 12, 18 and 24h after injection of sodium taurocholate model 6 time points, the detection of brain water content, blood brain Barrier permeability changes were detected by Western blotting and semi-quantitative reverse transcription PCR detection of brain tissue AQP4 protein and m RNA expression. Results: The expression of AQP4 protein and m RNA in brain tissue were up-regulated 6 h after injury, and then increased at 9, 12, 18 and 24 h (P <0.05). AQP4 protein and m RNA expression and brain water content were positively correlated (P <0.05). CONCLUSION: The decreased blood-brain barrier function and the increased expression of AQP4 protein and m RNA in brain tissue of ANP rats may be closely related to the formation and development of cerebral edema.