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目的:探讨miR-21反义寡核苷酸(AS-miR-21)对内皮型一氧化氮合酶(eNOS)基因调节的分子机制及血管内皮细胞增殖相关转录因子AP1作用机制的可能性。方法:应用MTT方法检测细胞增殖抑制率;细胞划痕实验检测细胞迁移能力;Transwell侵袭小室模型分别检测各组细胞侵袭能力的变化;Western blot检测miR-21表达相关情况及细胞核转录因子eNOS和AP1表达情况。结果:AS-miR-21能抑制人脐静脉内皮细胞(HUVECs)生长,促进其凋亡;AS-miR-21对HUVECs迁移和侵袭具有抑制作用;AS-miR-21干预下HUVECs的eNOS和AP1蛋白表达明显减少。结论:AS-miR-21显著抑制eNOS和AP1蛋白表达,转录因子AP1在ASmiR-21对eNOS的表达调节中起重要作用。
OBJECTIVE: To investigate the molecular mechanism of miR-21 antisense oligonucleotide (AS-miR-21) on the regulation of endothelial nitric oxide synthase (eNOS) gene and the possible mechanism of action of vascular endothelial cell proliferation-related transcription factor AP1. Methods: MTT assay was used to detect the cell proliferation inhibition rate. Cell scratch assay was used to detect cell migration ability. Transwell invasive chamber model was used to detect the invasion ability of cells. Western blot was used to detect the expression of miR-21 and eNOS and AP1 Express the situation. Results: AS-miR-21 could inhibit the growth of human umbilical vein endothelial cells (HUVECs) and promote its apoptosis. AS-miR-21 inhibited the migration and invasion of HUVECs. The expression of eNOS and AP1 Protein expression was significantly reduced. Conclusion: AS-miR-21 significantly inhibits the expression of eNOS and AP1, and transcription factor AP1 plays an important role in the regulation of eNOS expression by ASmiR-21.