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目的 :探讨肺缺血再灌注损伤的病理学改变及中药 814的干预作用。方法 :7只杂种犬随机分两组 :①急性肺缺血再灌注组 (3只 ) :结扎左主肺动脉 2 4h后恢复血流 4h ;②中药 814干预组 (4只 ) :再灌注前予中药 814腹腔注射 ,应用光镜和电镜等病理检测方法观察缺血期、再灌注期、中药 814干预后肺组织病理形态学改变 ,计算受损肺泡百分率。结果 :①急性肺缺血再灌注组 :缺血期 ,左肺散在出血 ,部分肺泡上皮细胞及肺血管内皮细胞胞质肿胀、线粒体空泡化、内质网扩张 ;同例对照右肺基本正常。再灌注期 ,左肺出血加重 ,肺间质及广泛肺泡腔内有水肿液 ,受损肺泡百分率显著增加 ,肺泡上皮细胞和肺血管内皮细胞肿胀、线粒体空泡化及嵴溶解加重 ,可见细胞坏死 ;同例对照右肺大致正常或散在小灶性肺泡腔内有水肿和出血 ,少数肺泡上皮细胞和肺血管内皮细胞轻度线粒体肿胀和空泡化。②中药 814干预组 :再灌注期左肺受损肺泡百分率显著减少 ,少数肺泡上皮细胞和肺血管内皮细胞线粒体轻度肿胀和空泡化。结论 :急性缺血后再灌注肺组织病理学形态显示比较明显的损伤 ,中药 814对再灌注肺损伤有一定的实验防治作用
Objective: To explore the pathological changes of lung ischemia-reperfusion injury and the intervention of Chinese medicine 814. METHODS: Seven mongrel dogs were randomly divided into two groups: 1 acute lung ischemia-reperfusion group (3 rats): left main pulmonary artery was ligated for 24 hours after 24 hours; 2 Chinese medicine 814 intervention group (4 rats): before reperfusion Intraperitoneal injection of Chinese medicine 814, light microscope and electron microscopy were used to observe the pathological changes of lung tissue in ischemic phase, reperfusion phase, and traditional Chinese medicine 814 intervention, and calculate the percentage of damaged alveoli. RESULTS: 1Acute pulmonary ischemia-reperfusion group: During the ischemic phase, the left lung scattered in the hemorrhage, some alveolar epithelial cells and pulmonary vascular endothelial cells were swollen, mitochondria vacuolized, and the endoplasmic reticulum was expanded; the right lung was normal as compared with the control group. . During the reperfusion period, left lung hemorrhage aggravated, edema fluid was found in interstitial lung and extensive alveolar spaces, the percentage of damaged alveoli increased significantly, swelling of alveolar epithelial cells and pulmonary vascular endothelial cells, vacuolation of mitochondria, and dissolution of hemorrhoids aggravated. ; The same example control right lung is generally normal or scattered in the focal alveolar cavity with edema and hemorrhage, a small number of alveolar epithelial cells and pulmonary vascular endothelial cells mild mitochondrial swelling and vacuolization. 2 Traditional Chinese Medicine 814 intervention group: The percentage of damaged alveoli in the left lung was significantly reduced during reperfusion, and a few of the alveolar epithelial cells and pulmonary vascular mitochondria were slightly swollen and vacuolated. Conclusion : The histopathology of lung tissue after acute ischemic reperfusion shows more obvious damage. Chinese medicine 814 has certain preventive and therapeutic effects on reperfusion lung injury.