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为获得高诱导率的花生毛状根诱导条件,同时探究不同浓度的萘乙酸(NAA)和2,4二氯苯氧乙酸(2,4-D)对花生毛状根生长和白藜芦醇含量的影响。研究了不同发根农杆菌菌株和不同乙酰丁香酮浓度对花生毛状根诱导率的影响;以固体MS培养基为对照,分别添加不同浓度的NAA和2,4-D,培养花生毛状根,探究对毛状根生长和白藜芦醇含量的影响。实验显示花生毛状根诱导率可达到92.3%。花生毛状根随着NAA的浓度升高,生物量同对照相比较无显著差异,各浓度的NAA之间也无明显差异;与对照相比较,白藜芦醇含量显著增高,呈显著差异,但各浓度的NAA之间白藜芦醇的含量无明显差异。添加2,4-D形成的生物量明显偏低,白藜芦醇含量与添加NAA组无差别。以A600为0.8的发根农杆菌R1601菌液侵染花生叶片,在含50μmol/L乙酰丁香酮的MS培养基培养时,可以高效的诱导得到花生毛状根;NAA可以作为一种外源激素提高花生毛状根中白藜芦醇的含量。
In order to obtain high induction rate of hairy roots of peanut, the effects of different concentrations of NAA and 2,4-D on the growth of peanut hairy roots and the effects of resveratrol Effect of content. The effects of different Agrobacterium rhizogenes strains and different concentrations of acetosyringone on the induction rate of hairy roots of peanut were studied. NAA and 2,4-D with different concentrations were added to the solid MS medium, , To explore the hairy root growth and resveratrol content. Experiments show that the induction rate of hairy roots of peanuts can reach 92.3%. Compared with the control, there was no significant difference in the biomass of hairy roots of peanut with NAA concentration. There was no significant difference in NAA between the two groups. Compared with the control, the content of resveratrol was significantly increased, However, there was no significant difference in the content of resveratrol between NAA of different concentrations. Addition of 2,4-D formed biomass was significantly lower, resveratrol content and added NAA group no difference. The peanut leaves were inoculated with Agrobacterium rhizogenes R1601 with A600 of 0.8, and the hairy roots of peanut were efficiently induced when cultured in MS medium containing 50 μmol / L acetosyringone. NAA could be used as an exogenous hormone Improve the content of resveratrol in the hairy root of peanut.