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目的 :研究利用脱氧葡萄糖的荧光标记类似物 6 - [N - (7-硝基苯基 - 2 -氧杂 - 1,3-重氮盐 - 4-基 )氨基 ]- 2 -脱氧葡萄糖 (2 -NBDG)进行荧光显像的可行性。方法 :本试验利用了 3只雄性小鼠。鼠 1饥饿 16h后在室内光照条件下由尾静脉注入 0 .0 2 6mg/ 0 .1ml的 2 -NBDG ,然后该小鼠保持室内光照直至处死。鼠 2正常进食 ,在完全黑暗的环境下由尾静脉注入 0 .0 2 6mg/ 0 .1ml的 2 -NBDG ,然后该小鼠保存在黑暗环境下直至处死。鼠 3在室内光照条件下由尾静脉注入 0 .1ml的生理盐水 ,然后该小鼠保存在室内光照环境直至处死。 3只小鼠均在注射后 30min处死 ,其中鼠 2是在黑暗环境下处死 ,鼠 1和鼠 3在室内光线下处死。处死后分离 3只小鼠的大脑并进行水平方向切片 ,层厚 10 μm。我们选用每只小鼠视皮质部位的 7个切片 ,在一个大视野的CCD系统下进行荧光显像。结果 :显像结果表明 ,在接受 2 -NBDG注射的 2只鼠 (鼠 1和鼠 2 )的大脑内可以见到明显的荧光。而未接受 2 -NBDG注射的鼠 3大脑内则未见任何荧光。鼠 1双侧视皮质对应部位荧光强度与周围皮质无明显差别 ,而鼠 2双侧视皮质对应部位荧光强度则略低于周围皮质。对鼠 1和鼠 2大脑荧光图像的定量分析表明 ,鼠 1视皮质部位的平均荧光强度明显高于鼠 2 (P
OBJECTIVE: To study the effects of deoxyglucose on fluorescence intensity of deoxyglucose (GlcNAc), a fluorescent marker analogue of deoxyglucose, 2 - [N - (7 - nitrophenyl - 2 - oxa - -NBDG) for fluorescence imaging feasibility. Methods: Three male mice were used in this experiment. Mouse 1 was starved for 16 h and then injected with 0.226mg / 0.1ml of 2-NBDG from the tail vein in a room light condition, and then the mouse was kept in room light until sacrifice. Mice 2 were fed normally and were injected with 0.026 mg / 0.1 ml of 2-NBDG from the tail vein in a completely dark environment, which mice were then stored in the dark until sacrificed. Mice 3 were injected with 0.1 ml of saline from the caudal vein under room light conditions, and then the mice were kept in an indoor light environment until sacrifice. All three mice were sacrificed 30 min after injection, in which mouse 2 was sacrificed in darkness and mouse 1 and mouse 3 sacrificed in room light. After sacrifice, the brains of three mice were separated and sliced in the horizontal direction at a layer thickness of 10 μm. We selected seven sections of the visual cortex of each mouse for fluorescence imaging under a large field of view CCD system. RESULTS: The imaging results showed that significant fluorescence was seen in the brains of 2 rats injected with 2-NBDG (mouse 1 and mouse 2). In rats not receiving 2-NBDG injection, no fluorescence was observed in the 3 brains. There was no significant difference in the fluorescence intensity between the corresponding sites of the bilateral visual cortex of the rat 1 and the surrounding cortex, while the fluorescence intensity of the corresponding site of the bilateral visual cortex of the rat 2 was slightly lower than that of the surrounding cortex. Quantitative analysis of the fluorescence images of rat 1 and mouse 2 brain showed that the average fluorescence intensity of the visual cortex of mouse 1 was significantly higher than that of mouse 2 (P