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目的研究妊娠晚期母鼠脱水对成年子代大鼠脑肾素-血管紧张素系统(RAS)及其对水盐代谢调控的影响。方法妊娠母鼠随机分成对照组(Con)及脱水组(WD),脱水组于妊娠晚期脱水3 d,检测其成年子代鼠(5个月)血氧分压(PO2)、二氧化碳分压(PCO2)、pH值、血钠离子(Na+)和钾离子(K+)浓度及血浆渗透压(Osm)水平;检测其前脑血管紧张素受体(AT1R、AT2R)蛋白表达的水平,并记录成年子代鼠脑室给予血管紧张素Ⅱ(AngⅡ)后2 h内氯化钠(1.8%NaCl)和水的摄取量。结果妊娠晚期母鼠脱水3 d后,其成年子代鼠血PO2、PCO2、pH值、血Na+和K+浓度及Osm水平的差异均无统计学意义(均P>0.05);子代雌、雄大鼠前脑组织中AT1R蛋白表达水平均显著升高(均P<0.01),AT2R蛋白水平在子代雌鼠明显升高(P<0.05),雄鼠的差异无统计学意义(P>0.05);但雄鼠前脑的AT1/AT2蛋白表达比率明显升高(P<0.05),雌鼠的差异无统计学意义;子代雌、雄大鼠脑室给予AngⅡ后2 h内摄盐量的差异无统计学意义(P>0.05),子代雄鼠摄水量显著增多(P<0.01)。结论妊娠晚期母鼠脱水可引起其成年子代水盐代谢的改变,该变化可能与前脑血管紧张素受体表达上调有关。
Objective To investigate the effects of dehydration of maternal rats during late pregnancy on the brain renin-angiotensin system (RAS) and its regulation of water and salt metabolism in adult offsprings. Methods Pregnant females were randomly divided into control group (Con) and dehydration group (WD). Dehydration group was dehydrated for 3 days in the third trimester of gestation. The PO2 and PO2 (PCO2), pH value, Na + and K + concentration and plasma osmotic pressure (Osm) were measured. The protein expression of AT1R and AT2R in forebrain was detected and recorded Sodium chloride (1.8% NaCl) and water uptake within 2 h after intraventricular administration of angiotensin II (Ang II) in offspring rats. Results There was no significant difference in PO2, PCO2, pH, blood Na + and K + concentrations and Osm levels in the third trimester of pregnancy after 3 days of dehydration in the third trimester of pregnancy (all P> 0.05) (P <0.01). AT2R protein level was significantly increased in offspring female rats (P <0.05), but there was no significant difference in male rats (P> 0.05) ; However, the ratio of AT1 / AT2 protein expression in the frontal gyrus of male rats was significantly increased (P <0.05), while there was no significant difference in female rats. There was no significant difference in salt intake between the male and female rats within 2 h Statistical significance (P> 0.05), offspring male rats increased significantly (P <0.01). Conclusion Dehydration of maternal rats in the second trimester of pregnancy may cause changes of water and salt metabolism in their offspring, which may be related to the upregulation of angiotensin receptor expression in the forebrain.